Effects of phage endolysin SAL200 combined with antibiotics on Staphylococcus aureus infection.

Effects of phage endolysin SAL200 combined with antibiotics on Staphylococcus aureus infection. Antimicrob Agents Chemother. 2018 Jul 23;: Authors: Kim NH, Park WB, Cho JE, Choi YJ, Choi SJ, Jun SY, Kang CK, Song KH, Choe PG, Bang JH, Kim ES, Park SW, Kim NJ, Oh MD, Kim HB Abstract Phages and its derivatives are increasingly reconsidered for the treatment of bacterial infections, due to rising antibiotic resistance. We assessed the anti-staphylococcal effect of the endolysin SAL200 in combination with standard-of-care (SOC) antibiotics. SAL200 activity combined with SOC antibiotics was assessed in vitro by checkerboard and time-kill assays, and in vivo with murine bacteremia and Galleria mellonella infection models. SAL200 reduced SOC antibiotic MICs and showed a ≥3-log10CFU/mL reduction of S. aureus within 30 minutes in time-kill assays. Combinations of SAL200 and SOC antibiotics achieved a sustained decrease of >2-log10CFU/mL. SAL200 significantly lowered blood bacterial density within 1 hour by > 1 log10CFU/mL in bacteremic mice (P<0.05 vs untreated mice), and SAL200 and SOC antibiotic combinations achieved the lowest levels of bacteremia. Bacterial density in splenic tissue at 72 h post-infection was lowest in mice treated with SAL200 and SOC antibiotic combinations. SAL200 combined with SOC antibiotics also improved Galleria mellonella larvae survival at 96 h post-infection.The combination of phage endolysin SAL200 wi...
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research