Simultaneous determination of tryptophan, kynurenine, kynurenic acid and two monoamines in rat plasma by HPLC-ECD/DAD

Publication date: 5 September 2018Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 158Author(s): Ting-ting Du, Ting Cui, Hong-mei Qiu, Nian-ru Wang, Dan Huang, Xin-hui JiangAbstractA high-performance liquid chromatography method with a diode array and an electrochemical detection (HPLC-ECD/DAD) was developed to determine the levels of tryptophan (TRP), kynurenine (KYN), kynurenic acid (KYA), 5-hydroxytryptamine (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) in rat plasma. The prepared samples were separated on a BDS column (4.6 mm × 250 mm, 5 mm) with column oven temperature of 25 °C. The mobile phase consisted of 5% acetonitrile and a buffer solution, which contained 25 mmol/L sodium acetate and 0.01 mmol/L EDTA, adjusting pH to 4.5 with acetic acid, and it was pumped at a flow-rate of 1.0 mL/min. KYN and KYA were measured by a variable wavelength detector at wavelengths 360 nm and 333 nm respectively, TRP and vanillic acid (as IS) both were measured at 280 nm. Determination of 5-HT and 5-HIAA was accomplished at the electrochemical working potential of 700 mV. Total run time was 14 min. Several parameters of the developed method were validated including linearity, accuracy precision, and stability. The results showed the established method had good LOD and separation for all of the five compounds and IS in the biological matrix. The method is simple, fast, economical and accurate. The analytical method and the results c...
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research

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Publication date: Available online 16 November 2019Source: Journal of Contextual Behavioral ScienceAuthor(s): Alan K. Davis, Frederick S. Barrett, Roland R. GriffithsAbstractPrior research has shown that acute subjective psychedelic effects are associated with both spontaneous and intended changes in depression and anxiety. Psychedelics are also theorized to produce increases in psychological flexibility, which could explain decreases in depression and anxiety following a psychedelic experience. Therefore, the present cross-sectional survey study sought to examine whether psychological flexibility mediated the relationship...
Source: Journal of Contextual Behavioral Science - Category: Psychiatry & Psychology Source Type: research
Depression is the most common mental health problem in the world. → Support PsyBlog for just $4 per month. Enables access to articles marked (M) and removes ads. → Explore PsyBlog's ebooks, all written by Dr Jeremy Dean: Accept Yourself: How to feel a profound sense of warmth and self-compassion The Anxiety Plan: 42 Strategies For Worry, Phobias, OCD and Panic Spark: 17 Steps That Will Boost Your Motivation For Anything Activate: How To Find Joy Again By Changing What You Do
Source: PsyBlog | Psychology Blog - Category: Psychiatry & Psychology Authors: Tags: Depression subscribers-only Source Type: blogs
Publication date: Available online 16 November 2019Source: Progress in Neuro-Psychopharmacology and Biological PsychiatryAuthor(s): Mijung Yeom, Sora Ahn, Ju-Young Oh, Song-Yi Kim, Hyangsook Lee, Dae-Hyun Hahm, Hi-Joon ParkAbstractClinically, it has been reported that atopic dermatitis (AD) has been linked with negative emotional problems such as depression and anxiety, thereby reducing the quality of life, but little is known about the molecular mechanism that underlies AD-associated emotional impairments. We sought to determine whether AD could induce anxiety- and depressive-like symptoms in mice and to identify pertinen...
Source: Progress in Neuro Psychopharmacology and Biological Psychiatry - Category: Psychiatry Source Type: research
“Parents cannot opt out of knowing that I exist.” That was one of the comments as UNISON’s LGBT conference discussed inclusive education and it was met with loud applause. Speaker James Anthony is from the West Midlands, where protests outside Parkfield Primary School in Birmingham, against the teaching of the No Outsiders scheme, have made national headlines. The protests at Parkfield have been primarily among Muslim parents, who have been misled about the nature of the teaching. No Outsiders “isn’t about sex education,” stressed Mr Anthony. “It’s storybooks showing that par...
Source: UNISON meat hygiene - Category: Food Science Authors: Tags: Article News education Source Type: news
Conclusions: Similar to previous studies, our results suggest that patients suffering from CLBP differ with regard to the magnitude of mental burden and the presence of physical impairment. These differences ascertain the need for precise targeting of treatment for CLBP. Inpatient pain centers therefore should offer different multimodal therapy pathways and integrate a meaningful triage, taking into account the multifaceted nature of CLBP based on sophisticated knowledge about forms, differences, and relationships among the biopsychosocial components of CLBP. PMID: 31728134 [PubMed - in process]
Source: GMS German Medical Science - Category: General Medicine Tags: Ger Med Sci Source Type: research
Publication date: Available online 15 November 2019Source: SeizureAuthor(s): Hyo Jae Kim, Ji-Ye Jeon, Hyun-Woo Kim, Sang-Ahm LeeAbstractPurposeWe compared the Neurological Disorders Depression Inventory for Epilepsy (NDDI-E) with the Patient Health Questionnaire-9 (PHQ-9) according to different antiepileptic drug (AED) loads in people with epilepsy (PWE).MethodsDepression and suicidality were assessed with the Mini International Neuropsychiatric Interview (MINI), the NDDI-E, and the PHQ-9. A receiver operating characteristic (ROC) curve analysis was used. Sensitivity and specificity of the NDDI-E and PHQ-9 were compared between an AED load
Source: Seizure - Category: Neurology Source Type: research
Authors: Ferreira LP, Pinheiro CF, Fernandes NA, Ferreira CN PMID: 31730304 [PubMed - in process]
Source: The Primary Care Companion for CNS Disorders - Category: Primary Care Tags: Prim Care Companion CNS Disord Source Type: research
This study examined the relationship between parental uncertainty and parent proxy reports of HRQOL in newly diagnosed children with cancer. METHODS: A longitudinal descriptive approach was used to examine the relationship between parental uncertainty and parent proxy reports of HRQOL in 55 parent-child dyads. Parental trait anxiety, depression, and perceived social support were evaluated as potential predictor variables. FINDINGS: Parents of newly diagnosed children with cancer reported lower parent proxy HRQOL scores. Parental uncertainty was prevalent and affected parents' perceptions of HRQOL three months fol...
Source: Clinical Journal of Oncology Nursing - Category: Nursing Authors: Tags: Clin J Oncol Nurs Source Type: research
Publication date: Available online 15 November 2019Source: Journal of Psychiatric ResearchAuthor(s): Brandon Nichter, Moira Haller, Sonya Norman, Robert H. PietrzakAbstractConverging evidence suggests that veterans with co-occurring PTSD/MDD represent a high-risk group for poor mental health compared to those with PTSD alone. To date, however, little is known about the specific factors that may increase vulnerability for and help buffer risk for comorbid PTSD/MDD. The purpose of this study was to provide a population-based characterization of sociodemographic, risk, and protective variables associated with comorbid PTSD/MD...
Source: Journal of Psychiatric Research - Category: Psychiatry Source Type: research
In conclusion, morphine administration during adolescence induced persistent changes in negative affect and brain toxicity selectively in male rats, suggesting female rats were resilient to these harmful effects. Given the widespread availability and use of opiate-based painkillers, the interplay between addiction, analgesia and emotional behaviors, and since adolescents and young adult humans are the age group with the highest abuse potential, these results add to the current literature by reporting distinct sex-specific opioid actions when administered in adolescence.
Source: Neuroscience Letters - Category: Neuroscience Source Type: research
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