A fast method to reprogram and CRISPR/Cas9 gene editing from erythroblasts

Publication date: Available online 4 July 2018 Source:Stem Cell Research Author(s): Uirá Souto Melo, Felipe de Souza Leite, Silvia Costa, Carla Rosenberg, Mayana Zatz An efficient one-step procedure to reprogram fibroblasts into human induced pluripotent stem cells (hiPSC) and perform CRISPR/Cas9 gene editing simultaneously was recently reported. Here we show that such simultaneous reprogramming and gene editing can be efficiently done from erythroblasts. We successfully obtained human induced pluripotent stem cells colonies together with in frame and out of frame CAPN1 mutations in one or both alleles. We did not identify off-targets in edited cell lines. The entire process, from blood collection to mutated hiPSC took approximately 5 weeks, a much shorter period than standard multi-step methodologies using fibroblasts. Noteworthy, blood drawing is a less invasive procedure than a skin biopsy.
Source: Stem Cell Research - Category: Stem Cells Source Type: research