Isolation and characterization of porcine PILRB gene and its alternative splicing variants.

In this study, we obtained the cDNA (V1) of its encoding gene, PILRB, and three alternative splicing (AS) variants (V2-4). The complete coding sequence of V1 was 621 bp long encoding a polypeptide of 206 aa. Compared with V1, V2 and V3 were formed by exon-skipping in the 3'-untranslated region (UTR), while V4 was formed by alternative 3' splice site of exon 3, resulting in a premature termination codon, combined with exon skipping in the 3'-UTR. Expression profile analysis showed that all the isoforms were most abundant in the spleen, and V1 was strongly induced by poly(I:C). Furthermore, the transcription of V1 altered with the increasing age and differed between species. Exon skipping in the 3'-UTR of V2 and V3 down-regulated expression of the luciferase reporter gene, and hence presumably of the PILRB gene, while V4 was subjected to nonsense-mediated mRNA decay. Additionally, five novel splicing patterns were detected using the minigene approach, indicating complex AS of porcine PILRB. These results will help to reveal the role of PILRβ in the host immune response using pig models, and will facilitate the breeding of pigs resistant to viral diseases through molecular breeding methods.
PMID: 29879501 [PubMed - as supplied by publisher]

Source: Gene - June 4, 2018 Category: Genetics & Stem Cells Authors: Yang XQ, Jing XY, Zhang CX, Song YF, Liu D Tags: Gene Source Type: research