GSE104206 Assessing eRNAs associated with a cytokine-sensing mammary super-enhancer

Contributors : Chaochen Wang ; Tyler Kuhns ; Christian Reinbold ; Michaela Willi ; Lothar HennighausenSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusEnhancers are transcription factor platforms that also bind RNA polymerase II and generate enhancer RNAs (eRNA). Although eRNAs have been suggested as predictors of enhancer activities and possibly key components facilitating transcription, there is little genetic evidence to support this. We address the biology of eRNAs in vivo and investigate eRNA patterns, expression levels and possible functions within a mammary-specific super-enhancer that is composed of three units with distinct transcriptional capacities. We show that eRNA levels do not correspond with the activities of their respective enhancer units. However, changes in eRNA expression upon deletion of individual enhancer units reflect the change in overall super-enhancer activity. These data provide genetic evidence that eRNA levels are not a reliable readout of individual enhancers, but they predict superenhancer activity in the absence of constituent elements.

Source: GEO: Gene Expression Omnibus - June 1, 2018 Category: Genetics & Stem Cells Tags: Genome binding/occupancy profiling by high throughput sequencing Mus musculus Source Type: research