A novel collagen glycopeptide, Pro-Hyp-CONH-GlcN, stimulates cell proliferation and hyaluronan production in cultured human dermal fibroblasts

This study investigated the effects of POGlcN on dermal fibroblast growth, hyaluronan production, and the underlying mechanism. The results suggest that the plasma digestion-resistant POGlcN stimulated the proliferation of cultured human dermal fibroblasts in a time- and concentration-dependent manner with optimal performance at 200 nmol/mL and 6 days. Comparable to Pro-Hyp, POGlcN increased the cell number to 1.5-fold and HA production to 1.4-fold of control. Edu labeling-assisted cell cycle analysis revealed that POGlcN modulated the cell cycle of fibroblasts by arresting more cells in DNA synthesis phase. Moreover, POGlcN up-regulated both hyaluronan synthase-2 and -3 gene transcriptions to 1.8-fold and 2.0-fold of control, respectively. The results implied the potency of POGlcN for improving skin health and skin regeneration. Graphical abstract
Source: Journal of Functional Foods - Category: Nutrition Source Type: research