GSE92436 Expression data from progesterone receptor knockout versus heterozygous mouse ovaries: cumulus oocyte complexes

Contributors : Lisa K Akison ; Darryl L Russell ; Rebecca L RobkerSeries Type : Expression profiling by arrayOrganism : Mus musculusOvulation requires sequential molecular events and structural remodeling in the ovarian follicle for the successful release of a mature oocyte capable of being fertilised. Critical to this process is progesterone receptor (PGR), a transcription factor highly yet transiently expressed in granulosa cells of preovulatory follicles. Progesterone receptor knockout (PRKO) mice are anovulatory, with a specific and complete defect in follicle rupture. Therefore, this model was used to examine the critical molecular and biochemical mechanisms necessary for successful ovulation.Although PGR is not expressed in the cumulus cells or oocyte of the preovulatory cumulus oocyte complex (COC), it is well known that the COC responds to the cascade of gene expression changes that occurs in preovulatory granulosa cells. We used microarrays to identify putative ‘ovulation’ genes in preovulatory COCs at a time when PGR expression is maximal in granulosa cells (eCG + 8h hCG) and the preovulatory COC and follicle are undergoing the final changes necessary for successful ovulation.

Source: GEO: Gene Expression Omnibus - April 20, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by array Mus musculus Source Type: research