GSE99295 Downregulated d-limonene transgenic orange trees with increased linalool content showed resistance to Phyllosticta citricarpa

Contributors : Ana Rodr íguez ; Vanessa Kava ; Lorena Latorre-García ; Geraldo J da Silva Jr ; Rosana G Pereira ; Chirlei Glienke ; Lisandra S Ferreira-Maba ; Antonio Vicent ; Takehiko Shimada ; Leandro PeñaSeries Type : Expression profiling by arrayOrganism : Citrus ; Citrus sinensisTerpene volatiles play an important role in the interactions among specialized pathogens and fruits. Citrus Black Spot (CBS), caused by the fungus Phyllosticta citricarpa, is associated with losses in different citrus-growing areas worldwide. The pathogen may infect the fruit for 20-24 weeks after petal fall but the typical hard spot symptoms appear when the fruit become almost mature, as consequence of fungal colonization and induction of cell lyses around essential oil cavities. D-limonene represents about 95% of total oil gland content in mature orange fruit. We have used here d-limonene synthase downregulated orange fruit generated via an antisense (AS) approach to investigate whether d-limonene decrease content in peel oil glands may affect fruit interaction with P. citricarpa compared to that of empty-vector (EV) controls. AS fruit showed enhanced resistance to the fungus compared to EV ones. Because of d-limonene decreased content, an over-accumulation of linalool and other monoterpene alcohols was found in AS compared to EV fruit. A global gene expression analysis at 2h and 8d after inoculation with P. citricarpa revealed activation of defence responses in AS fruit, via the upre...

Source: GEO: Gene Expression Omnibus - April 13, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by array Citrus Citrus sinensis Source Type: research