miR-122 Regulates LHR Expression in Rat Granulosa Cells by Targeting Insig1 mRNA.

We present evidence showing that mRNA and protein levels of Insig1 undergo a time-dependent increase following the treatment of rat granulosa cells with FSH, which leads to a decrease in LRBP levels. Furthermore, overexpression of miR-122 using an adenoviral vector (AdmiR-122) abolished FSH-induced increases in Insig1 mRNA and protein. We further confirmed the role of Insig1 by showing that inhibition of Insig1 using a specific siRNA (siInsig1) prior to FSH treatment resulted in the abrogation of LHR upregulation. Silencing of Insig1 also reversed FSH-mediated decreases in SREBP and LRBP activation. These results show that decreased levels of miR-122 increase Insig1 and suppress SREBP processing in response to FSH stimulation of rat granulosa cells. PMID: 29579170 [PubMed - as supplied by publisher]
Source: Endocrinology - Category: Endocrinology Authors: Tags: Endocrinology Source Type: research