GSE90738 Circular RNAs are super abundant in cervical tumor and plasma detected by high throughput microarray [cervical_cancer_mRNA]

Contributors : Shasha Li ; Shuaishuai Teng ; Junquan Xu ; Guannan Su ; Jianqing Zhao ; Wenyan Qin ; Qianyong Zhu ; Dong WangSeries Type : Expression profiling by arrayOrganism : Homo sapiensCircular RNAs (circRNAs) are a new class of endogenous and regulatory non-coding RNAs, but their expressions in tumor and plasma are largely unknown. Here, our study firstly suggested that microarray should be more efficient than RNA sequencing for circRNA profiling. Then, we detected ~80,000 circRNAs expressed in cervical tumors and matched normal tissues by microarray, and ~23,000 of them are differently expressed. The numbers of up- and down-regulated circRNAs during tumorigenesis are almost equal. In addition, we discovered that the expression of different circRNA isoforms from the same linear RNA also different. Strikingly, as much as ~18,000 circRNAs could be robustly detected in plasmas, and ~8,000 circRNAs show different expression after surgery of tumor removal. Altogether, taking advantage of the huge number of circRNAs detected in tumor and plasma, we provide strong evidence for circRNA expression, regulation and potential clinical application as non-invasive biomarkers.

Source: GEO: Gene Expression Omnibus - March 1, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research