Evaluation of two commercial Real-Time PCR kits, A. fumigatus ® Bio-Evolution and MycoGENIE® A. fumigatus for Aspergillus DNA detection in Bronchoalveolar Lavage Fluid in patients with invasive pulmonary aspergillosis

Invasive pulmonary aspergillosis (IPA) is a common complication of immunosuppression. Rapid diagnosis using molecular techniques is essential to improve patient survival. PCR techniques are promising in enhancing Aspergillus detection in blood and respiratory samples. Here, we evaluate for the first time the performances of two commercial Real-Time PCR kits, the A. fumigatus ® Bio-Evolution and the MycoGENIE® A. fumigatus for the detection of A. fumigatus DNA in broncho-alveolar lavage (BAL) from patients with and without IPA.

Source: Journal of Molecular Diagnostics - February 19, 2018 Category: Pathology Authors: Julie Denis, Faezeh Forouzanfar, Raoul Herbrecht, Elise Toussaint, Romain Kessler, Marcela Sabou, Ermanno Candolfi, Val érie Letsher-Bru Tags: Regular Article Source Type: research