Identification and characterization of microRNA in the lung tissue of pigs with different susceptibilities to PCV2 infection

In this study, high-throughput sequencing was performed to determine the abundance and differential expression of miRNA in lung tissues from PCV2-infected and PCV2-uninfected LW and YL pigs . In total, 295 known and 95 novel miRNA were identified, and 23 known and 25 novel miRNA were significantly differentially expressed in the PCV2-infected vs. PCV2-uninfected LW pigs and/or the PCV2-infected vs. PCV2-uninfected YL pigs. The expression levels ofssc-miR-122,ssc-miR-192,ssc-miR-451,ssc-miR-486, andssc-miR-504 were confirmed by quantitative real-time PCR (qRT-PCR). Analysis of the potential targets of the four up-regulated miRNA (i.e.,ssc-miR-122,ssc-miR-192,ssc-miR-451 andssc-miR-486) identified pathways and genes that may be important for disease resistance. Among the up-regulated miRNA,ssc-miR-122 can repress the protein expression and viral DNA replication of PCV2 and down-regulate the expression of thenuclear factor of activated T-cells 5 (NFAT5) andaminopeptidase puromycin sensitive (NPEPPS) by binding to their 3 ′ untranslated region (3′UTR) in PK15 cells. Therefore,ssc-miR-122 may indirectly suppress PCV2 infection by targeting genes related to the host immune system, such asNFAT5 andNPEPPS.
Source: Veterinary Research - Category: Veterinary Research Source Type: research