Molecules, Vol. 23, Pages 374: Inhibitory Effect of Flavonolignans on the P2Y12 Pathway in Blood Platelets

Molecules, Vol. 23, Pages 374: Inhibitory Effect of Flavonolignans on the P2Y12 Pathway in Blood Platelets Molecules doi: 10.3390/molecules23020374 Authors: Michal Bijak Rafal Szelenberger Angela Dziedzic Joanna Saluk-Bijak Adenosine diphosphate (ADP) is the major platelet agonist, which is important in the shape changes, stability, and growth of the thrombus. Platelet activation by ADP is associated with the G protein-coupled receptors P2Y1 and P2Y12. The pharmacologic blockade of the P2Y12 receptor significantly reduces the risk of peripheral artery disease, myocardial infarction, ischemic stroke, and vascular death. Recent studies demonstrated the inhibition of ADP-induced blood platelet activation by three major compounds of the flavonolignans group: silybin, silychristin, and silydianin. For this reason, the aim of the current work was to verify the effects of silybin, silychristin, and silydianin on ADP-induced physiological platelets responses, as well as mechanisms of P2Y12-dependent intracellular signal transduction. We evaluated the effect of tested flavonolignans on ADP-induced blood platelets’ aggregation in platelet-rich plasma (PRP) (using light transmission aggregometry), adhesion to fibrinogen (using the static method), and the secretion of PF-4 (using the ELISA method). Additionally, using the double labeled flow cytometry method, we estimated platelet vasodilator-stimulated phosphoprotein (VASP) phosphorylation. We demonstrated a dose-depende...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research