Endometrial stromal cell attachment and matrix homeostasis in abdominal wall endometriomas

AbstractSTUDY QUESTIONHow does progesterone alter matrix remodeling in abdominal wall endometriomas compared with normal endometrium?SUMMARY ANSWERProgesterone may prevent attachment of endometrial cells to the abdominal wall, but does not ameliorate abnormal stromal cell responses of abdominal wall endometriomas.WHAT IS KNOWN ALREADYMenstruation is a tightly orchestrated physiologic event in which steroid hormones and inflammatory cells cooperatively initiate shedding of the endometrium. Abdominal wall endometriomas represent a unique form of endometriosis in which endometrial cells inoculate fascia or dermis at the time of obstetrical or gynecologic surgery. Invasion of endometrium into ectopic sites requires matrix metalloproteinases (MMPs) for tissue remodeling but endometrium is not shed externally.STUDY DESIGN SIZE, DURATIONObservational study in 14 cases and 19 controls.PARTICIPANTS /MATERIALS, SETTING, METHODSTissues and stromal cells isolated from 14 abdominal wall endometriomas were compared with 19 normal cycling endometrium using immunohistochemistry, quantitative PCR, gelatin zymography and cell attachment assays.P values< 0.05 were considered significant and experiments were repeated in at least three different cell preps to provide scientific rigor to the conclusions.MAIN RESULTS AND THE ROLE OF CHANCEThe results indicate that MMP2 and MMP9 are not increased by TGF β1 in endometrioma stromal cells. Although progesterone prevents attachment of endometrioma c...

Source: Human Reproduction - December 28, 2017 Category: Reproduction Medicine Source Type: research