Detection of Gallibacterium anatis by TaqMan fluorescent quantitative PCR.

Detection of Gallibacterium anatis by TaqMan fluorescent quantitative PCR.
Avian Pathol. 2017 Dec 15;:1-25
Authors: Huangfu H, Xu W, Wang H, Dong Q, Guo H, Sun Y, Li Y, Gao W, Wang W, Zhang J, Shi J, Pan H, Li C, Wang L
Abstract
To better understand the prevalence of Gallibacterium anatis in different poultry species, a rapid and accurate method was developed to detect G. anatis using a TaqMan fluorescent quantitative polymerase chain reaction (qPCR). Specific primers and a TaqMan probe were designed based on the reference gtxA gene sequence. The qPCR standard curve showed a good linear relationship, and the method showed good reproducibility, sensitivity, and specificity, indicating its suitability for G. anatis identification and quantitative analysis. Comparison of the detection results in 160 clinical swab samples showed that the detection rate (54.4%) of the qPCR for G. anatis was better than that of two conventional methods: gyrB gene-based qPCR for G. anatis (51.9%) and culture-based identification (34.4%). G. anatis was detected in layer chicken (77.3%), Silkie chicken (72.7%), and duck (27.1%) with relatively high detection rates, whereas dove (8.8%), and quail (3.0%), showed lower detection rates, indicating the different prevalence of G. anatis in different fowl species.
PMID: 29243936 [PubMed - as supplied by publisher]

Source: Avian Pathology - December 15, 2017 Category: Pathology Authors: Huangfu H, Xu W, Wang H, Dong Q, Guo H, Sun Y, Li Y, Gao W, Wang W, Zhang J, Shi J, Pan H, Li C, Wang L Tags: Avian Pathol Source Type: research