Oxidative stress and Na,K-ATPase activity differential regulation in brainstem and forebrain of Wistar Audiogenic Rats may lead to increased seizure susceptibility.

Oxidative stress and Na,K-ATPase activity differential regulation in brainstem and forebrain of Wistar Audiogenic Rats may lead to increased seizure susceptibility.
Brain Res. 2017 Dec 07;:
Authors: Parreira GM, Resende MDA, Garcia IJP, Sartori DB, Umeoka EHL, Godoy LD, Garcia-Cairasco N, Barbosa LA, Santos HL, Tilelli CQ
Abstract
The Wistar Audiogenic Rat (WAR) is a well-characterized seizure-prone, inbred rodent strain that, when acutely stimulated with high-intensity sounds, develops brainstem-dependent tonic-clonic seizures that can evolve to limbic-like, myoclonic (forebrain) seizures when the acoustic stimuli are presented chronically (audiogenic kindling). In order to investigate possible mechanisms underlying WAR susceptibility to seizures, we evaluated Na,K-ATPase activity, Ca-ATPase activity, Mg-ATPase activity, lipid membrane composition and oxidative stress markers in whole forebrain and whole brainstem samples of naïve WAR, as compared to samples from control Wistar rats. We also evaluated the expression levels of α1 and α3 isoforms of Na,K-ATPase in forebrain samples. We observed increased Na,K-ATPase activity in forebrain samples and increased oxidative stress markers (lipid peroxidation, glutathione peroxidase and superoxide dismutase) in brainstem samples of WAR. The Ca-ATPase activity, Mg-ATPase activity, lipid membrane composition and expression levels of α1 and α3 isoforms of Na,K-ATPase were unaltered. In vi...

Source: Brain Research - December 7, 2017 Category: Neurology Authors: Parreira GM, Resende MDA, Garcia IJP, Sartori DB, Umeoka EHL, Godoy LD, Garcia-Cairasco N, Barbosa LA, Santos HL, Tilelli CQ Tags: Brain Res Source Type: research