Functional characterization of O ‐methyltransferases used to catalyse site‐specific methylation in the post‐tailoring steps of pradimicin biosynthesis

ConclusionsPdmF and PdmT were involved in 11‐O‐ and 7‐O‐methylations of the benzo[α]naphthacenequinone moiety of pradimicin, respectively. Methylation of the C‐7 hydroxyl group precedes methylation of the C‐11 hydroxyl group in pradimicin biosynthesis.
Significance and Impact of the StudyThis is the first reported demonstration of the functions of PdmF and PdmT for regiospecific O‐methylation, which contributes to better understanding of the post‐PKS modifications in pradimicin biosynthesis as well as to rational engineering of the pradimicin biosynthetic machinery.

Source: Journal of Applied Microbiology - December 11, 2017 Category: Microbiology Authors: J.W. Han, B.G. Ng, J.K. Sohng, Y.J. Yoon, G.J. Choi, B.S. Kim Tags: Original Article Source Type: research