Evaluation of two commercial real-time PCR assays for detection of carbapenemase genes in Enterobacteriaceae.
Evaluation of two commercial real-time PCR assays for detection of carbapenemase genes in Enterobacteriaceae.
J Med Microbiol. 2017 Oct 16;:
Authors: Ashkenazi Hoffnung L, Burdelova E, Adler A
Abstract
The implementation of PCR for the detection of carbapenemase genes enables rapid results with significant epidemiological implications. Two commercial real-time PCR assays, the Hylabs Hy-CRE and Sacace MDR MBL+KPC/OXA, were evaluated for the detection of the genes blaKPC, blaVIM, blaNDM, blaIMP and blaOXA-48-carbapenemasein a collection of 96 carbapenem-resistant Enterobacteriaceae strains with different resistant mechanisms. Both assays exhibited excellent diagnostic performance, with 100 % sensitivity and specificity.
PMID: 29034864 [PubMed - as supplied by publisher]
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Ashkenazi Hoffnung L, Burdelova E, Adler A Tags: J Med Microbiol Source Type: research