Mechanism of the effect of saikosaponin on atherosclerosis in  vitro is based on the MAPK signaling pathway.

Mechanism of the effect of saikosaponin on atherosclerosis in vitro is based on the MAPK signaling pathway. Mol Med Rep. 2017 Oct 03;: Authors: Yang L, Liu J, Qi G Abstract The present study aimed to investigate the effects of saikosaponin on oxidized low‑density lipoprotein (ox‑LDL)‑induced human umbilical vein endothelial cell (HUVEC) injury and apoptosis, and examine the involvement of the mitogen‑activated protein kinase (MAPK) signaling pathway. The viability and apoptosis of HUVECs were detected using an MTT assay and flow cytometry. ELISA analysis was applied to measure the levels of tumor necrosis factor (TNF)‑α and interleukin (IL)‑6 cytokines. Nuclear factor (NF)‑κB p65 nuclear translocation was observed using immunofluorescence staining. The levels of intercellular adhesion molecule 1 and vascular cell adhesion molecule‑1 were detected using reverse transcription‑polymerase chain reaction analysis. The phosphorylation of B‑cell lymphoma 2 (Bcl‑2), Bcl‑2‑associated X protein (Bax), caspase‑3 p38, c‑Jun N‑terminal kinase (JNK) and extracellular signal‑regulated kinase (ERK)1/2 were detected using western blot analysis. The results revealed that saikosaponin increased the viability of the HUVECs and decreased the early‑stage apoptotic rate of the HUVECs induced by ox‑LDL. The expression levels of inflammatory cytokines in the injured vascular endothelial cells were decreased, the expr...
Source: Molecular Medicine Reports - Category: Molecular Biology Tags: Mol Med Rep Source Type: research