Regulation of Inflammatory Phenotype in Macrophages by a Diabetes-Induced Long Non-coding RNA.

Regulation of Inflammatory Phenotype in Macrophages by a Diabetes-Induced Long Non-coding RNA.
Diabetes. 2014 Jul 9;
Authors: Reddy MA, Chen Z, Park JT, Wang M, Lanting L, Zhang Q, Bhatt K, Leung A, Wu X, Putta S, Sætrom P, Devaraj S, Natarajan R
Abstract
The mechanisms by which macrophages mediate the enhanced inflammation associated with diabetes complications are not completely understood. We used RNA-seq to profile the transcriptome of bone marrow macrophages isolated from diabetic db/db mice and identified 1648 differentially expressed genes compared to control db/+ mice. Data analyses revealed that diabetes promoted a pro-inflammatory, pro-fibrotic and dysfunctional alternatively activated macrophage phenotype possibly via transcription factors involved in macrophage function. Notably, diabetes altered levels of several long non-coding RNAs (lncRNAs). Because, the role of lncRNAs in diabetic complications is unknown, we further characterized the function of lncRNA E330013P06 which was up-regulated in macrophages from db/db and diet-induced insulin resistant type-2 diabetic mice, but not from type-1 diabetic mice. It was also up-regulated in monocytes from type-2 diabetes patients. E330013P06 was also increased along with inflammatory genes, in mouse macrophages treated with high glucose and palmitic acid. E330013P06 overexpression in macrophages induced inflammatory genes, enhanced responses to inflammatory signals, and increa...

Source: Diabetes - July 9, 2014 Category: Endocrinology Authors: Reddy MA, Chen Z, Park JT, Wang M, Lanting L, Zhang Q, Bhatt K, Leung A, Wu X, Putta S, Sætrom P, Devaraj S, Natarajan R Tags: Diabetes Source Type: research