Post-transcriptional and metabolic regulation of erythropoiesis

Cellular differentiation requires highly coordinated gene expression through transcriptional and post-transcriptional mechanisms. Advances in high-throughput sequencing enabled genome-scale quantification of mRNA for gene expression, yet the extent to which changes in mRNA are translated to protein remains unclear. Here we measured genome-wide protein and mRNA expression in human primary fetal liver and adult bone marrow-derived CD34+ hematopoietic stem/progenitor cells (HSPCs) and differentiated erythroid precursors (proerythroblasts or ProEs) by mass-spectrometry-based quantitative proteomics and RNA-seq analysis, respectively.
Source: Experimental Hematology - Category: Hematology Authors: Source Type: research