GSE99252 Human virus-derived small RNAs can confer antiviral immunity in mammals

Contributors : Yang Qiu ; Yanpeng Xu ; Yao Zhang ; Hui Zhou ; Yong-Qiang Deng ; Xiao-Feng Li ; Meng Miao ; Qiang Zhang ; Bo Zhong ; Yuanyang Hu ; Fu-Chun Zhang ; Ligang Wu ; Cheng-Feng Qin ; Xi ZhouSeries Type : Non-coding RNA profiling by high throughput sequencingOrganism : Homo sapiensRNA interference (RNAi) functions as a potent antiviral immunity in plants and invertebrates, however whether RNAi plays antiviral roles in mammals remains unclear. Here, using human enterovirus 71 (HEV71) as a model, we showed HEV71 3A protein as an authentic viral suppressor of RNAi during viral infection. When the 3A-mediated RNAi suppression was impaired, the mutant HEV71 readily triggered the production of abundant HEV71-derived small RNAs with canonical siRNA properties in cells and mice. These virus-derived siRNAs were produced from viral dsRNA replicative intermediates in a Dicer-dependent manner, loaded into AGO, and were fully active in degrading cognate viral RNAs. Recombinant HEV71 deficient in 3A-mediated RNAi suppression was significantly restricted in human somatic cells and mice, whereas Dicer-deficiency rescued HEV71 infection independently of type I interferon response. Thus, RNAi can function as an antiviral immunity, which is induced and suppressed by a human virus, in mammals.

Source: GEO: Gene Expression Omnibus - August 1, 2017 Category: Genetics & Stem Cells Tags: Non-coding RNA profiling by high throughput sequencing Homo sapiens Source Type: research