The Effect of Ultraviolet B on Fibrillin-1 and Fibrillin-2 in Human Non-pigmented Ciliary Epithelial Cells In Vitro.

The Effect of Ultraviolet B on Fibrillin-1 and Fibrillin-2 in Human Non-pigmented Ciliary Epithelial Cells In Vitro.
Acta Histochem Cytochem. 2017 Jun 26;50(3):105-109
Authors: Shiroto Y, Terashima S, Hosokawa Y, Oka K, Isokawa K, Tsuruga E
Abstract
The ciliary zonules link the lens to the ciliary body in the eye, controlling the thickness of the lens for focusing through their characteristic elasticity. The ciliary zonules are composed of oxytalan fibers. Physiological or pathological damage to the ciliary zonules, including exposure to ultraviolet (UV)-A and UV-B components, can lead to lens dislocation. However, no studies have shown whether UV affects the ciliary zonule. Here, we assessed the effects of UV light on human nonpigmented ciliary epithelial cells (HNPCECs). HNPCECs were cultured for 4 weeks, and expression of fibrillin-1 and fibrillin-2 was confirmed. In control cultures (0 mJ/cm(2)), some fibrillin-1-positive fibers were merged with fibrillin-2. After UV-A irradiation, the appearance of both fibrillin-1- and fibrillin-2-positive fibers was unchanged. However, after UV-B irradiation, fibrillin-1-positive fibers became thin at an irradiation level of 100 mJ/cm(2), and the fiber structure became amorphous at 150 mJ/cm(2). Fibrillin-2-positive fibers lost their continuity and disappeared after being exposed to 150 mJ/cm(2) UV-B. UV-B irradiation did not affect cell viability, possibly because of the sensitivity of fibril...

Source: Acta Histochemica et Cytochemica - June 26, 2017 Category: Biochemistry Authors: Shiroto Y, Terashima S, Hosokawa Y, Oka K, Isokawa K, Tsuruga E Tags: Acta Histochem Cytochem Source Type: research