Codependency of Metabolism and Epigenetics Drives Cancer Progression: A Review.
Abstract Cancer is widely considered to be a set of genetic diseases that are currently classified by tissue and cell type of origin and, increasingly, by its molecular characteristics. This latter aspect is based primarily upon oncogene gains, tumor suppressor losses, and associated transcriptional profiles. However, cancers are also characterized by profound alterations in cellular metabolism and epigenetic landscape. It is particularly noteworthy that cancer-causing genomic defects not only activate cell cycle progression, but regulate the opportunistic uptake and utilization of nutrients, effectively enabling ...
Source: Acta Histochemica et Cytochemica - February 28, 2020 Category: Biochemistry Authors: Masui K, Harachi M, K Cavenee W, S Mischel P, Shibata N Tags: Acta Histochem Cytochem Source Type: research

Incremental Growth Lines in Mouse Molar Dentin Represent 8-hr Ultradian Rhythm.
Abstract Rhythmic incremental growth lines occur in dental hard tissues of vertebrates, and dentinogenesis in rodent incisors is suggested to be controlled by the 24-hr circadian clock. Rodent incisors continue to grow throughout the animal's life; however, similar to human teeth, rodent molars stop growing after crown formation. This similarity suggests that the mouse molar is an excellent model to understand the molecular mechanisms underlying growth of human teeth. However, not much is known about the rhythmic dentinogenesis in mouse molars. Here, we investigated the incremental growth lines in mouse molar dent...
Source: Acta Histochemica et Cytochemica - December 27, 2019 Category: Biochemistry Authors: Ono R, Koike N, Inokawa H, Tsuchiya Y, Umemura Y, Yamamoto T, Kanamura N, Yagita K Tags: Acta Histochem Cytochem Source Type: research

Localization and Accumulation Studies of Dacomitinib in Rat Intestines and Skin by Immunohistochemistry.
This report is the first to elucidate the localization and accumulation of dacomitinib in the rat intestine and skin and should be valuable during efforts to clarify the mechanism dacomitinib-induced diarrhea or skin toxicities. PMID: 32001948 [PubMed] (Source: Acta Histochemica et Cytochemica)
Source: Acta Histochemica et Cytochemica - December 27, 2019 Category: Biochemistry Authors: Yamamoto Y, Saita T, Oka A, Kataoka H, Shin M Tags: Acta Histochem Cytochem Source Type: research

Hypothalamic Kisspeptin Expression in Hyperandrogenic Female Rats and Aging Rats.
Abstract Hypothalamic kisspeptin neurons stimulate gonadotropin-releasing hormone (GnRH) and luteinizing hormone (LH) release. Kisspeptin neurons in the anteroventral periventricular nucleus (AVPV) of rats induce an LH surge for ovulation, and those in the arcuate nucleus (ARC) regulate pulsatile LH secretion for follicle development and spermatogenesis. Dysfunction of kisspeptin neurons thus reduces the reproductive function. This review focuses on the effect of androgen or aging on kisspeptin expression in rats. Although androgen directly suppresses ARC kisspeptin neurons in female rats, the AVPV kisspeptin neur...
Source: Acta Histochemica et Cytochemica - October 29, 2019 Category: Biochemistry Authors: Iwata K, Kunimura Y, Ozawa H Tags: Acta Histochem Cytochem Source Type: research

Reversible Conversion among Subtypes of Salivary Gland Duct Cells as Identified by Production of a Variety of Bioactive Polypeptides.
Abstract Four major kallikreins (mK1, mK22, mK9, and mK13) were identified in the mouse submandibular gland (SMG). mK1, a true tissue kallikrein, was used as a protein marker to identify different types of SMG granular convoluted tubule (GCT) cells along with epidermal growth factor (EGF), nerve growth factor (NGF), and renin. Kallikrein mK1 was localized in a very small number (~5%) of GCT cells, which were scattered throughout the GCT, indicating that the majority of GCT cells are mK1-negative. Among mK1-positive cells, particularly strong signals were observed in a small number of narrow cells, recognized as sl...
Source: Acta Histochemica et Cytochemica - August 30, 2019 Category: Biochemistry Authors: Kurabuchi S, Yao C, Chen G, Hosoi K Tags: Acta Histochem Cytochem Source Type: research

The Effect of Estrogen on Hepatic Fat Accumulation during Early Phase of Liver Regeneration after Partial Hepatectomy in Rats.
In conclusion, these results indicated that estrogen and ERα might play an important role in fat accumulation related to FAT/CD36 during early phase of rat liver regeneration. PMID: 31592200 [PubMed] (Source: Acta Histochemica et Cytochemica)
Source: Acta Histochemica et Cytochemica - August 30, 2019 Category: Biochemistry Authors: Srisowanna N, Choijookhuu N, Yano K, Batmunkh B, Ikenoue M, Nhat Huynh Mai N, Yamaguchi Y, Hishikawa Y Tags: Acta Histochem Cytochem Source Type: research

Modulation of αvβ3 Integrin via Transactivation of β3 Integrin Gene on Murine Bone Marrow Macrophages by 1,25(OH)2D3, Retinoic Acid and Interleukin-4.
Modulation of αvβ3 Integrin via Transactivation of β3 Integrin Gene on Murine Bone Marrow Macrophages by 1,25(OH)2D3, Retinoic Acid and Interleukin-4. Acta Histochem Cytochem. 2019 Aug 30;52(4):77-83 Authors: Kitazawa S, Haraguchi R, Kohara Y, Kitazawa R Abstract The interleukin (IL)-4, 1,25(OH)2D3 and retinoic acid, increase surface expression of functional integrin αvβ3 on murine osteoclast precursors. All three agonists stimulate transcription of the β3 gene, leading to increased steady-state levels of mRNA this protein. By contrast, mRNA levels of αv remain un...
Source: Acta Histochemica et Cytochemica - August 30, 2019 Category: Biochemistry Authors: Kitazawa S, Haraguchi R, Kohara Y, Kitazawa R Tags: Acta Histochem Cytochem Source Type: research

Long-term Pilocarpine Treatment Improves Salivary Flow in Irradiated Mice.
Abstract Radiation therapy for head and neck cancer frequently causes salivary gland dysfunction. Pilocarpine is a clinically approved and effective drug that induces saliva secretion, thereby keeping the oral mucosa moist and reducing discomfort in patients, but the effect is transient. We expected that this drug also has beneficial long-term effects that maintain the integrity of salivary glands by reducing, for instance, apoptosis. Here, we examined the effects of long-term pilocarpine administration in irradiated mice. The results indicated that long-term pilocarpine administration significantly improved saliv...
Source: Acta Histochemica et Cytochemica - June 25, 2019 Category: Biochemistry Authors: Taniguchi A, Susa T, Kogo H, Iizuka-Kogo A, Yokoo S, Matsuzaki T Tags: Acta Histochem Cytochem Source Type: research

Immunohistochemical Localization of RNase 7 in Normal and Inflamed Oral Epithelia and Salivary Glands.
Abstract RNase 7 is a skin-derived antimicrobial peptide expressed in various epithelial tissues. It is upregulated by stimulation with microbes and pro-inflammatory cytokines. Herein, we examined the expression levels of RNase 7 in tissues from normal and inflamed oral epithelia and salivary glands via immunohistochemistry. RNase 7 was expressed mainly in the surface layers of the parakeratinized and orthokeratinized oral epithelium. In addition, it was strongly and weakly expressed in oral lichen planus and radicular cysts, respectively. RNase 7 was constitutively expressed in salivary glands, particularly in th...
Source: Acta Histochemica et Cytochemica - April 25, 2019 Category: Biochemistry Authors: Neopane P, Paudel D, Yoshida K, Raj Adhikari B, Morikawa T, Onishi A, Hiraki D, Uehara O, Sato J, Nishimura M, Chiba I, Abiko Y Tags: Acta Histochem Cytochem Source Type: research

The HDAC Inhibitor, SAHA, Combined with Cisplatin Synergistically Induces Apoptosis in Alpha-fetoprotein-producing Hepatoid Adenocarcinoma Cells.
In this study, we investigated the anticancer effect of SAHA in combination with cisplatin in VAT-39 cells, a newly established HAC cell line. Cell viability and apoptosis were examined by MTT assay, flow cytometry and TUNEL assay. Expression of H3S10, cleaved caspase-3, Bax, and Bcl-2 were evaluated by immunohistochemistry and western blotting. AFP levels were examined in VAT-39 cells and culture medium. Combined treatment with cisplatin and SAHA efficiently inhibited cell proliferation and decreased cell viability. Apoptotic cells, but not necrotic cells, were significantly increased following the combined treatment, and...
Source: Acta Histochemica et Cytochemica - February 28, 2019 Category: Biochemistry Authors: Kyaw MTH, Yamaguchi Y, Choijookhuu N, Yano K, Takagi H, Takahashi N, Synn Oo P, Sato K, Hishikawa Y Tags: Acta Histochem Cytochem Source Type: research

Immunohistochemical Mapping of Bcl9 Using Two Antibodies that Recognize Different Epitopes Is Useful to Characterize Juvenile Development of Hepatocellular Carcinoma in Myanmar.
In this study, we examined the expression pattern of Bcl9 immunohistochemically, using two anti-Bcl9 antibodies; one was a conventional polyclonal-antibody (anti-Bcl9ABC) against amino acid no.800-900 of human-Bcl9, while the other (anti-Bcl9BIO) was against amino acid no.50-200, covering Pygopus-binding sites of Bcl9. Immunohistochemistry using anti-Bcl9BIO demonstrated distinctive staining in the cytoplasm, while the anti-Bcl9ABC signal was detected in both cytoplasm and nuclei of HCC cells, reflecting different states of Bcl9 function because Pygopus-binding to Bcl9 is essential to exert its function together with &beta...
Source: Acta Histochemica et Cytochemica - February 28, 2019 Category: Biochemistry Authors: Soe MT, Shibata Y, Win Htun M, Abe K, Soe K, Win Than N, Lwin T, Phone Kyaw M, Koji T Tags: Acta Histochem Cytochem Source Type: research

The Role of the Wnt Signaling Pathway in Upper Jaw Development of Chick Embryo.
Abstract Cleft lip with or without cleft palate (CLP) usually results from a failure of the medial nasal prominences to fuse with the lateral and maxillary prominences. This failure inhibits facial morphogenesis regulated by several major morphogenetic signaling pathways. We hypothesized that CLP results from the failure of the Wnt signaling pathway. To examine whether Wnt signaling can influences upper jaw development, we applied beads soaked with Dickkopf-1 (Dkk-1), Alsterpaullone (AL) or Wnt3a to the right side of the maxillary prominence of the chick embryo. The embryo showed a defect of the maxilla on the tre...
Source: Acta Histochemica et Cytochemica - February 28, 2019 Category: Biochemistry Authors: Shimomura T, Kawakami M, Tatsumi K, Tanaka T, Morita-Takemura S, Kirita T, Wanaka A Tags: Acta Histochem Cytochem Source Type: research

Immunohistochemistry for Anti-diabetes Drug, Alogliptin Using a Newly Prepared Monoclonal Antibody: Its Precise Localization in Rat Small Intestine.
Abstract Knowledge of time sequence of localization of drugs in cells and tissues of animals may help in developing a better understanding of the actual overall pharmacokinetics of the drugs. We produced monoclonal antibody (mAb) against alogliptin (AG), a dipeptidyl peptidase-4 (DPP-4) inhibitor, conjugated to BSA with N-(γ-maleimidobutyryloxy)-succinimide. The mAb was specific for AG and did not cross-react with sitagliptin, vancomycin or amoxicillin. The mAb enabled us to develop an immunohistochemical method for detecting the localization of AG in the rat small intestine. One hour after a single oral adm...
Source: Acta Histochemica et Cytochemica - February 28, 2019 Category: Biochemistry Authors: Yamamoto Y, Yamamoto Y, Saita T, Shin M Tags: Acta Histochem Cytochem Source Type: research

New Insight into the Role of Nitric Oxide Pathways in Pancreas.
Abstract Nitric oxide (NO) is generated by a family of enzymes termed NO synthases (NOS) that convert L-arginine to NO and citrulline. The role of NO as an important biological mediator and recognition of the pathophysiological significance of superoxides/NO interaction has led to an intensive research and development of therapies based on the interception of the NO signaling cascade in the pancreatitis course. However, the presence and localization of the NO-generating enzymes in various organs including pancreas are subject to controversy. We assumed that this controversy might reflect rather the diversity of ex...
Source: Acta Histochemica et Cytochemica - December 20, 2018 Category: Biochemistry Authors: Buchwalow I, Schnekenburger J, Samoilova V, Boecker W, Neumann J, Tiemann K Tags: Acta Histochem Cytochem Source Type: research

Stem-like Human Breast Cancer Cells Initiate Vasculogenic Mimicry on Matrigel.
Abstract Vasculogenic mimicry (VM), referring to vasculogenic structures lined by tumor cells, can be distinguished from angiogenesis, and is responsible for the aggressiveness and metastatic potential of tumors. HCC1937/p53 cells were derived from triple-negative breast cancer (TNBC), and used to investigate the roles of breast cancer stem cells (CSCs) in the formation of VM. HCC1937/p53 cells formed mesh-like structures on matrigel culture in which expression of VM-related genes, vascular endothelial (VE)-cadherin, matrix metalloproteinase (MMP)-2 and MMP-9 was confirmed by droplet digital polymerase chain react...
Source: Acta Histochemica et Cytochemica - December 20, 2018 Category: Biochemistry Authors: Izawa Y, Kashii-Magaribuchi K, Yoshida K, Nosaka M, Tsuji N, Yamamoto A, Kuroyanagi K, Tono K, Tanihata M, Imanishi M, Onishi M, Sakiyama M, Inoue S, Takahashi R Tags: Acta Histochem Cytochem Source Type: research

Collapsin Response Mediator Protein 1, a Novel Marker Protein for Differentiated Odontoblasts.
Abstract We previously reported that the terminal differentiation of odontoblasts was inhibited in Runx2 transgenic {Tg(Col1a1-Runx2)} mice under the control of the 2.3-kb Col1a1 promoter. Odontoblasts in Tg(Col1a1-Runx2) mice lose their characteristic long cellular processes, and show marked reductions in the protein levels of markers for odontoblasts, such as dentin sialophosphoprotein, nestin, and microtubule-associated protein tau (Mapt). We herein demonstrated that collapsin response mediator protein 1 (CRMP1), a neuronal phosphoprotein that participates in various aspects of neuronal development, was specifi...
Source: Acta Histochemica et Cytochemica - December 20, 2018 Category: Biochemistry Authors: Miyazaki T, T Baba T, Mori M, Komori T Tags: Acta Histochem Cytochem Source Type: research

Development of Chemical Tools for Imaging of Fe(II) Ions in Living Cells: A Review.
Abstract Intracellular labile iron is an iron species which is not or weakly bound to proteins and depicts an important effect on homeostatic regulation in cells. An excess or deficiency of iron can cause oxidative damage to key cellular biomolecules. The behavior and concentrations of labile iron are difficult to monitor, but the specific redox state of the Fe ions is relevant to the physiological and pathological properties that we would like to study. We have developed a series of turn-on type fluorescent probes that are highly selective to the labile Fe(II) ions, and we have tested their applications to cellul...
Source: Acta Histochemica et Cytochemica - October 31, 2018 Category: Biochemistry Authors: Hirayama T Tags: Acta Histochem Cytochem Source Type: research

Immunohistochemical Study of the Laminin α5 Chain and Its Specific Receptor, Basal Cell Adhesion Molecule (BCAM), in both Fetal and Adult Rat Pituitary Glands.
Immunohistochemical Study of the Laminin α5 Chain and Its Specific Receptor, Basal Cell Adhesion Molecule (BCAM), in both Fetal and Adult Rat Pituitary Glands. Acta Histochem Cytochem. 2018 Oct 31;51(5):145-152 Authors: Azuma M, Tsukada T, Inagaki T, Casmad F, Jindatip D, Tofrizal A, Maliza R, Batchuluun K, Syaidah R, Ohno N, Fujiwara K, Kikuchi M, Yashiro T Abstract Laminin, a major basement membrane protein, comprises three subunit chains: α, β, and γ chains. Among these chains, only the laminin α chain is capable of signaling via laminin receptors. Although laminin isof...
Source: Acta Histochemica et Cytochemica - October 31, 2018 Category: Biochemistry Authors: Azuma M, Tsukada T, Inagaki T, Casmad F, Jindatip D, Tofrizal A, Maliza R, Batchuluun K, Syaidah R, Ohno N, Fujiwara K, Kikuchi M, Yashiro T Tags: Acta Histochem Cytochem Source Type: research

Immunocytochemical Localization of Choline Acetyltransferase in the Microbat Visual Cortex.
Abstract The purpose of the present study was to investigate the organization of choline acetyltransferase (ChAT)-immunoreactive (IR) fibers in the visual cortex of the microbat, using standard immunocytochemistry and confocal microscopy. ChAT-IR fibers were distributed throughout all layers of the visual cortex, with the highest density in layer III and the lowest density in layer I. However, no ChAT-IR cells were found in the microbat visual cortex. ChAT-IR fibers were classified into two types: small and large varicose fibers. Previously identified sources of cholinergic fibers in the mammalian visual cortex, t...
Source: Acta Histochemica et Cytochemica - October 31, 2018 Category: Biochemistry Authors: Kim GH, Kim HG, Jeon CJ Tags: Acta Histochem Cytochem Source Type: research

Intranuclear Mobility of Estrogen Receptor: Implication for Transcriptional Regulation.
Abstract The estrogen receptor (ER) is a ligand-dependent transcription factor that has two subtypes: ERα and ERβ. ERs regulate transcription of estrogen-responsive genes through interactions with multiple intranuclear components, such as cofactors and the nuclear matrix. Live cell imaging using fluorescent protein-labeled ERs has revealed that ligand-activated ERs are highly mobile in the nucleus, with transient association with the DNA and nuclear matrix. Scaffold attachment factor B (SAFB) 1 and its paralogue, SAFB2, are nuclear matrix-binding proteins that negatively modulate ERα-mediated tran...
Source: Acta Histochemica et Cytochemica - August 29, 2018 Category: Biochemistry Authors: Matsuda KI, Hashimoto T, Kawata M Tags: Acta Histochem Cytochem Source Type: research

Label-free Molecular Imaging and Analysis by Raman Spectroscopy.
Abstract Raman scattering of a cell conveys the intrinsic information inherent to chemical structures of biomolecules. The spectroscopy of Raman scattering, or Raman spectroscopy, allows label-free and quantitative molecular sensing of a biological sample in situ without disruption. For the last five decades Raman spectroscopy has been widely utilized in biological research fields. However, it is just within the latest decade that molecular imaging and discrimination of living cells and tissues have become practically available. Here we overview recent progress in Raman spectroscopy and its application to life sci...
Source: Acta Histochemica et Cytochemica - June 26, 2018 Category: Biochemistry Authors: Kumamoto Y, Harada Y, Takamatsu T, Tanaka H Tags: Acta Histochem Cytochem Source Type: research

TRK-fused Gene Protein Localization Is Prominent in Serotonergic and Noradrenergic Cell Groups, and Some Lower Motor Neurons in the Corticospinal Tract of the Rat Brainstem.
In this study, we employed double immunohistochemistry to investigate the details of TFG localization patterns in monoaminergic and cholinergic neurons in the brainstem. Intense TFG immunoreactivity was observed in the dorsal raphe nucleus, the locus coeruleus, and the ventral horn of the spinal cord. TFG immunoreactivity was observed in some serotonergic neurons in all B1-B9 cell groups, and some noradrenergic neurons in all A1-A7 cell groups in the rat brainstem, while no immunoreactivity was observed in the dopaminergic neurons in A8-A10 cell groups. TFG immunoreactivity was observed in all ChAT-positive motor nuclei in...
Source: Acta Histochemica et Cytochemica - June 26, 2018 Category: Biochemistry Authors: Takeuchi S, Tooyama I Tags: Acta Histochem Cytochem Source Type: research

Biochemical and Morphological Characterization of a Guanine Nucleotide Exchange Factor ARHGEF9 in Mouse Tissues.
In this study, we prepared a specific polyclonal antibody against ARHGEF9, anti-ARHGEF9, and carried out expression analyses with mouse tissues especially brain. Western blotting analyses demonstrated tissue-dependent expression profiles of ARHGEF9 in the young adult mouse, and strongly suggested a role during brain development. Immunohistochemical analyses revealed developmental stage-dependent expression profiles of ARHGEF9 in cerebral cortex, hippocampus and cerebellum. ARHGEF9 exhibited partial localization at dendritic spines in cultured hippocampal neurons. From the obtained results, anti-ARHGEF9 was found to be a us...
Source: Acta Histochemica et Cytochemica - June 26, 2018 Category: Biochemistry Authors: Ibaraki K, Mizuno M, Aoki H, Niwa A, Iwamoto I, Hara A, Tabata H, Ito H, Nagata KI Tags: Acta Histochem Cytochem Source Type: research

Electron Microscopic Study Using Formalin-fixed, Paraffin-embedded Material, with Special Reference to Observation of Microbial Organisms and Endocrine Granules.
Abstract In the diagnostic pathology practice, specimens for electron microscopy (EM) are not necessarily handled and fixed under the ideal condition. In the present article, the author describes ultrastructural study using formalin-fixed and/or paraffin-embedded material. The fine morphologic preservation is often acceptable, particularly when small cubes are dug out of paraffin blocks. Particulate structures such as neuroendocrine granules and microbes are consistently observed even using paraffin sections. Paraffin sections signalized with silver particles in Grimelius and Grocott stains or diaminobenzidine pro...
Source: Acta Histochemica et Cytochemica - April 27, 2018 Category: Biochemistry Authors: Tsutsumi Y Tags: Acta Histochem Cytochem Source Type: research

Effect of Daily Light on c-Fos Expression in the Suprachiasmatic Nucleus under Jet Lag Conditions.
Abstract Jet-lag symptoms arise from temporal misalignment between the internal circadian clock and external solar time when traveling across multiple time zones. Light is known as a strong timing cue of the circadian clock. We here examined the effect of daily light on the process of jet lag by detecting c-Fos expression in the master clock neurons in the suprachiasmatic nucleus (SCN) under 8-hr phase-advanced jet lag condition. In WT mice, c-Fos-immunoreactivity was found at 1-2 hours on the first day after light/dark (LD) phase-advance. This induction was also observed on the second and third days, although the...
Source: Acta Histochemica et Cytochemica - April 27, 2018 Category: Biochemistry Authors: Chen Y, Yamaguchi Y, Suzuki T, Doi M, Okamura H Tags: Acta Histochem Cytochem Source Type: research

Morphological Analysis of Trafficking and Processing of Anionic and Cationic Liposomes in Cultured Cells.
Abstract Liposomes, artificial phospholipid vesicles, have been developed as a non-viral drug delivery system to allow contained agents to be efficiently delivered to target sites via systemic circulation. Liposomes have been used as a gene transfer tool with cultured cells; however, their precise trafficking and processing remain uncertain. Furthermore, liposomes with different surface charges are known to exhibit distinct properties. The purpose of the current study was to elucidate the intracellular trafficking and processing of liposomes with anionic and cationic surface charges from a morphological view point...
Source: Acta Histochemica et Cytochemica - April 27, 2018 Category: Biochemistry Authors: Tomori Y, Iijima N, Hinuma S, Ishii H, Takumi K, Takai S, Ozawa H Tags: Acta Histochem Cytochem Source Type: research

Changes in DNA Methylation of Oocytes and Granulosa Cells Assessed by HELMET during Folliculogenesis in Mouse Ovary.
In this study, we examined changes in the methylation level of CCGG and GATCG sites during mouse folliculogenesis in paraffin-embedded sections of mouse ovaries. For the purpose, we used a new method, histo endonuclease-linked detection of methylation sites of DNA (HELMET), designed to detect methylation sites of DNA with a specific sequence in a tissue section. Unlike the global level of DNA methylation, which was no change in immunohistochemical staining of 5-methylcytosine throughout folliculogenesis, we found that there were hypermethylation of CCGG and GATCG sites in most of the granulosa cells of tertiary follicles c...
Source: Acta Histochemica et Cytochemica - April 27, 2018 Category: Biochemistry Authors: Liu J, Zhang W, Wu Z, Dai L, Koji T Tags: Acta Histochem Cytochem Source Type: research

A Newly Defined Area of the Mouse Anterior Hypothalamus Involved in Septohypothalamic Circuit: Perifornical Area of the Anterior Hypothalamus, PeFAH.
In this study, we identified a new triangular-shaped area between the paraventricular hypothalamic nucleus (PVN) and the fornix area in the mouse anterior hypothalamus, which is enriched in chondroitin sulfate proteoglycans (CSPGs). We designated this region as the perifornical area of the anterior hypothalamus (PeFAH) based on its anatomical location. As evidenced by Nissl staining, the PeFAH was distinguishable as an area of relatively low density. Immunohistochemical and DNA microarray analyses indicated that PeFAH contains sparsely distributed calretinin-positive neurons and densely clustered enkephalin-positive neuron...
Source: Acta Histochemica et Cytochemica - February 27, 2018 Category: Biochemistry Authors: Horii-Hayashi N, Nishi M Tags: Acta Histochem Cytochem Source Type: research

Improved Serial Sectioning Techniques for Correlative Light-Electron Microscopy Mapping of Human Langerhans Islets.
In conclusion, the correlative imaging approach developed by us may be useful for examining ultrastructural features in combination with immunolocalisation of endocrine hormones in the same human pancreatic islets. PMID: 29622846 [PubMed] (Source: Acta Histochemica et Cytochemica)
Source: Acta Histochemica et Cytochemica - February 27, 2018 Category: Biochemistry Authors: Saitoh S, Ohno N, Saitoh Y, Terada N, Shimo S, Aida K, Fujii H, Kobayashi T, Ohno S Tags: Acta Histochem Cytochem Source Type: research

Estrogen Regulates Mitochondrial Morphology through Phosphorylation of Dynamin-related Protein 1 in MCF7 Human Breast Cancer Cells.
Abstract Estrogen affects mitochondrial function in various tissues, but the precise mechanism remains unclear. We, therefore investigated the effect on estrogen-regulated mitochondrial morphology by dynamin-related protein 1 (Drp1) and its Ser616-phosphorylated derivative (pDrp1Ser616) are involved in mitochondrial fission. MCF7 human breast cancer cells were treated with 17β-estradiol (E2), an estrogen receptor (ER) α and β antagonist (ICI 182, 780), an ERα antagonist (MPP), and an ERβ antagonist (PHTPP) for 24 hr. The expression of Drp1 and pDrp1Ser616 was analyzed by western blotting...
Source: Acta Histochemica et Cytochemica - February 27, 2018 Category: Biochemistry Authors: Oo PS, Yamaguchi Y, Sawaguchi A, Tin Htwe Kyaw M, Choijookhuu N, Noor Ali M, Srisowanna N, Hino SI, Hishikawa Y Tags: Acta Histochem Cytochem Source Type: research

The HDAC Inhibitor, SAHA, Prevents Colonic Inflammation by Suppressing Pro-inflammatory Cytokines and Chemokines in DSS-induced Colitis.
Abstract Inflammatory bowel disease (IBD) is an inflammatory disorder of the gastrointestinal tract that is caused by multiple factors, including dysfunction of the immune system and genetic and epigenetic alterations. Aberrant epigenetic regulation, especially histone acetylation, was found in biopsies from IBD patients and mouse models of colitis, suggesting that an epigenetic treatment approach may be useful for IBD therapy. Therefore, we investigated the effects of the histone deacetylase (HDAC) inhibitor, suberoylanilide hydroxamic acid (SAHA), in a mouse model of dextran sulfate sodium (DSS)-induced colitis....
Source: Acta Histochemica et Cytochemica - February 27, 2018 Category: Biochemistry Authors: Ali MN, Choijookhuu N, Takagi H, Srisowanna N, Nguyen Nhat Huynh M, Yamaguchi Y, Synn Oo P, Tin Htwe Kyaw M, Sato K, Yamaguchi R, Hishikawa Y Tags: Acta Histochem Cytochem Source Type: research

Expression and Dynamics of Podoplanin in Cultured Osteoblasts with Mechanostress and Mineralization Stimulus.
This study investigates the significance of the expression and dynamics of podoplanin in mechanostress and mineralization in cultured murine osteoblasts. Podoplanin increased in osteoblasts subjected to straining in non-mineralization medium, suggesting that the mechanostress alone is a podoplanin induction factor. In osteoblasts subjected to vertical elongation straining in the mineralization medium, the mRNA amounts of podoplanin, osteopontin, and osteocalcin were significantly larger than those in cells not subjected to straining, suggesting that mechanostress is the cause of a synergistic effect in the expression of th...
Source: Acta Histochemica et Cytochemica - February 27, 2018 Category: Biochemistry Authors: Takenawa T, Kanai T, Kitamura T, Yoshimura Y, Sawa Y, Iida J Tags: Acta Histochem Cytochem Source Type: research

Expression of Draxin in Lung Carcinomas.
Abstract Guidance molecules, such as Netrin-1, and their receptors have important roles in controlling axon pathfinding, modulate biological activities of various cancer cells, and may be a useful target for cancer therapy. Dorsal repulsive axon guidance protein (Draxin) is a novel guidance molecule that binds not only common guidance molecule receptors with Netrin-1, but also directly binds the EGF domain of Netrin-1 through a 22-amino-acid peptide (22aa). By immunostaining, Draxin was positively expressed in small cell carcinoma, adenocarcinoma (ADC), and squamous cell carcinoma of the lung. In addition, western...
Source: Acta Histochemica et Cytochemica - February 27, 2018 Category: Biochemistry Authors: Sato Y, Matsuo A, Kudoh S, Fang L, Hasegawa K, Shinmyo Y, Ito T Tags: Acta Histochem Cytochem Source Type: research

CXCL14-like Immunoreactivity Exists in Somatostatin-containing Endocrine Cells, and in the Lamina Propria and Submucosal Somatostatinergic Nervous System of Mouse Alimentary Tract.
Abstract In the present study, we investigated the distribution of CXCL14 immunoreactive endocrine cells and neurons in mouse alimentary tract by immunohistochemistry. CXCL14 immunoreactive endocrine cells were found as closed-type cells in the stomach and open-type cells in the small intestine. The immunostaining of these endocrine cells corresponded with that of the somatostatin-containing endocrine cells. Only a few CXCL14 immunoreactive endocrine cells were seen in the large intestine. CXCL14 immunoreactive fibers were observed in the muscular layer from the stomach to the rectum with most abundance in the rec...
Source: Acta Histochemica et Cytochemica - December 26, 2017 Category: Biochemistry Authors: Suzuki H, Yamada K, Matsuda Y, Onozuka M, Yamamoto T Tags: Acta Histochem Cytochem Source Type: research

CCN3 Expression Marks a Sulfomucin-nonproducing Unique Subset of Colonic Goblet Cells in Mice.
Abstract Intestinal goblet cells are characterized by their unique morphology and specialized function to secrete mucins. Although it is known that they are a heterogeneous population of cells, there have been few studies that relate the expression of a particular gene with functionally distinct subpopulations of intestinal goblet cells. Here we show that CCN3, a gene encoding a member of the CCN family proteins, is induced by inhibition of Notch signaling in colonic epithelial cells and expressed in goblet cells in mice. We demonstrate that CCN3 expression is confined to a subpopulation of goblet cells in the low...
Source: Acta Histochemica et Cytochemica - December 26, 2017 Category: Biochemistry Authors: Akiyama S, Mochizuki W, Nibe Y, Matsumoto Y, Sakamoto K, Oshima S, Watanabe M, Nakamura T Tags: Acta Histochem Cytochem Source Type: research

Novel Application of Loop-mediated Isothermal Amplification for Rapid Detection of Gene Translocation.
In this study, we developed novel application for detection of gene translocations using loop-mediated isothermal amplification (LAMP). We verified the amplified DNA products of echinoderm microtubule-associated protein-like 4 and anaplastic lymphoma kinase (EML4-ALK), synaptotagmin and synovial sarcoma, X breakpoint (SYT-SSX), and immunoglobulin heavy chain gene and B cell leukemia/lymphoma 2 (IgH/BCL2) by real-time PCR, agarose-gel electrophoresis, and the naked eye after the LAMP procedure. Fusion genes were detected in samples diluted 103 times within 60 min. Because of the advantages of rapid amplification, simple ope...
Source: Acta Histochemica et Cytochemica - December 26, 2017 Category: Biochemistry Authors: Matsuzaki I, Iguchi H, Mikasa Y, Morishita H, Okuda K, Nakaguchi K, Mori Y, Iwahashi Y, Warigaya K, Fujimoto M, Kojima F, Murata SI Tags: Acta Histochem Cytochem Source Type: research

Validation of Anti-CSP α, SNAP25, Tyrosine Hydroxylase, Ubiquitin, Cleaved Caspase 3, and pSer PKC Motif Antibodies for Utilization in Western Blotting.
In this study, we validated several commercial antibodies, including anti-CSPα, SNAP25, tyrosine hydroxylase, ubiquitin, cleaved caspase 3, and pSer PKC motif. Anti-CSPα, SNAP25, and tyrosine hydroxylase antibodies could detect their endogenous target proteins with some degree of cross-reactivity. Furthermore, clear SNAP25 staining was observed with SNAP25 antibody. Antibodies directed against ubiquitin, cleaved caspase 3, and pSer PKC motif could detect poly-ubiquitination, apoptosis, and phosphorylation, respectively. PMID: 29343881 [PubMed] (Source: Acta Histochemica et Cytochemica)
Source: Acta Histochemica et Cytochemica - December 26, 2017 Category: Biochemistry Authors: Shirafuji T, Ueyama T, Tanaka S, Hide I, Saito N, Sakai N Tags: Acta Histochem Cytochem Source Type: research

A Device for Ribbon Collection for Array Tomography with Scanning Electron Microscopy.
Abstract "Array tomography" is a method used to observe the fine structure of cells and tissues in a three-dimensional view. In this method, serial ultrathin sections in the ribbon state (ribbons) are mounted on a solid substrate and observed by scanning electron microscopy (SEM). The method may also be used in conjunction with post-embedding immunocytochemistry. However, it is difficult to mount many serial ribbons on a substrate manually. We developed an inexpensive laboratory-made device that mounts ribbons by pulling a nylon fishing line and lifting the substrate up from the water in a knife boat. Us...
Source: Acta Histochemica et Cytochemica - October 24, 2017 Category: Biochemistry Authors: Koike T, Kataoka Y, Maeda M, Hasebe Y, Yamaguchi Y, Suga M, Saito A, Yamada H Tags: Acta Histochem Cytochem Source Type: research

A New Electron Microscopic Method to Observe the Distribution of Phosphatidylinositol 3,4-bisphosphate.
Abstract Phosphatidylinositol 3,4-bisphosphate [PtdIns(3,4)P2] is a phosphoinositide that plays important roles in signal transduction, endocytosis, and cell migration among others. The intracellular distribution of PtdIns(3,4)P2 has mainly been studied by observing the distribution of GFP-tagged PtdIns(3,4)P2-binding protein domains in live cells and by labeling with anti-PtdIns(3,4)P2 antibody in fixed cell samples, but these methods only offer low spatial resolution results and may have pitfalls. In the present study, we developed an electron microscopic method to observe the PtdIns(3,4)P2 distribution using th...
Source: Acta Histochemica et Cytochemica - October 24, 2017 Category: Biochemistry Authors: Aktar S, Takatori S, Tsuji T, Orii M, Ohsaki Y, Cheng J, Fujimoto T Tags: Acta Histochem Cytochem Source Type: research

Morphological and Functional Analyses of the Tight Junction in the Palatal Epithelium of Mouse.
We examined the distribution pattern, constitutive proteins, and permeability of TJ in the stratified squamous epithelium of the palatal mucosa of mice. Ultrastructural observations based on the ultrathin section and freeze-fracture methods revealed that poorly developed TJs are located at the upper layer of the stratum granulosum. The positive immunofluorescence of occludin (OCD), claudin (CLD)-1 and -4 were localized among the upper layer of the stratum granulosum showing a dot-like distribution pattern. And CLD-1 and -4 were localized among the stratum spinosum and the lower part of stratum granulosum additionally showe...
Source: Acta Histochemica et Cytochemica - August 25, 2017 Category: Biochemistry Authors: Shiotsu N, Kawamoto T, Kawai M, Ikegame M, Torii Y, Sasaki H, Yamamoto T Tags: Acta Histochem Cytochem Source Type: research

Developmental Contribution of Wnt-signal-responsive Cells to Mouse Reproductive Tract Formation.
This study provides a precise outline for assessing the lineage relation between the reproductive tract and the cell fate of its primordia in a temporally regulated manner. PMID: 28928542 [PubMed] (Source: Acta Histochemica et Cytochemica)
Source: Acta Histochemica et Cytochemica - August 25, 2017 Category: Biochemistry Authors: Haraguchi R, Kitazawa R, Murashima A, Yamada G, Kitazawa S Tags: Acta Histochem Cytochem Source Type: research

The Effect of Ultraviolet B on Fibrillin-1 and Fibrillin-2 in Human Non-pigmented Ciliary Epithelial Cells In Vitro.
Abstract The ciliary zonules link the lens to the ciliary body in the eye, controlling the thickness of the lens for focusing through their characteristic elasticity. The ciliary zonules are composed of oxytalan fibers. Physiological or pathological damage to the ciliary zonules, including exposure to ultraviolet (UV)-A and UV-B components, can lead to lens dislocation. However, no studies have shown whether UV affects the ciliary zonule. Here, we assessed the effects of UV light on human nonpigmented ciliary epithelial cells (HNPCECs). HNPCECs were cultured for 4 weeks, and expression of fibrillin-1 and fibrillin...
Source: Acta Histochemica et Cytochemica - June 26, 2017 Category: Biochemistry Authors: Shiroto Y, Terashima S, Hosokawa Y, Oka K, Isokawa K, Tsuruga E Tags: Acta Histochem Cytochem Source Type: research

Expression of c-kit and Cytokeratin 5 in the Submandibular Gland after Release of Long-Term Ligation of the Main Excretory Duct in Mice.
Abstract Long-term submandibular duct obstruction is thought to cause irreversible atrophy and dysfunction of the submandibular gland. As an atrophic gland may be induced clinically by chronic or recurrent infection, it is generally removed surgically. However, the regenerative capacity of atrophic submandibular glands after long-term obstruction is not completely understood. We studied the regenerative capacity of the mouse submandibular duct using an aneurysm clip ligation model. We documented changes in the size, histologic structure and gene expression of the submandibular gland after 2 months of ligation, and...
Source: Acta Histochemica et Cytochemica - June 26, 2017 Category: Biochemistry Authors: Watanabe H, Takahashi H, Hata-Kawakami M, Tanaka A Tags: Acta Histochem Cytochem Source Type: research

Immunolocalization of Glutathione-Peroxidase (GPx1) in the Rat Adrenal Cortex: Correlation between Steroidogenesis and Lipid Peroxidation.
Abstract In order to confirm the relationship between glutathione-peroxidase (GPx1) and biological significance on steroidogenesis, we have studied the immunocytochemical localization of GPx1 in the rat adrenal cortical cells. GPx1 was observed not only in cytoplasm (cytosol GPx1) but in mitochondria (mitochondrial GPx1). The staining intensity was altered by the functional state of the adrenal cortical cells. Furthermore, cytosol- and mitochondrial-GPx1 was modified by lipoperoxidative damage in the adrenal cortical cells. Therefore, we proposed that the pattern of GPx1 staining should be a more sensitive and spe...
Source: Acta Histochemica et Cytochemica - April 27, 2017 Category: Biochemistry Authors: Murakoshi M, Osamura RY Tags: Acta Histochem Cytochem Source Type: research

Notch Signaling and Maintenance of SOX2 Expression in Rat Anterior Pituitary Cells.
Abstract After publication of reports describing the presence of stem/progenitor cells among non-hormone-producing cells in the pituitary, the mechanism responsible for proliferation and differentiation generated considerable interest. Several studies have suggested that Notch signaling is involved. In the present study, we examined the histochemical relationship between Notch signaling molecules and the transcription factor SOX2 in rat pituitary. Combined in situ hybridization and immunohistochemistry showed that Notch2 mRNA and SOX2 were co-expressed at embryonic day 14.5 in most cells in the adenohypophyseal pr...
Source: Acta Histochemica et Cytochemica - April 27, 2017 Category: Biochemistry Authors: Batchuluun K, Azuma M, Fujiwara K, Yashiro T, Kikuchi M Tags: Acta Histochem Cytochem Source Type: research

Monoclonal Suncus Antibodies: Generation of Fusion Partners to Produce Suncus-Suncus Hybridomas.
Abstract We used suncus (Suncus murinus; house musk shrew) to generate partner cells for cell fusion to produce suncus monoclonal antibodies. Suncus are insectivores that are genetically distant to rodents, and recognize antigens and epitopes that are not immunogenic in mice and rats, which are the animals most commonly used in basic life science research and from which monoclonal antibodies are usually produced. To date, monoclonal antibodies from suncus have not been generated due to the lack of a plasmacytoma fusion partner. To obtain suncus plasmacytoma cell lines suitable as a cell fusion partner, we injected...
Source: Acta Histochemica et Cytochemica - April 27, 2017 Category: Biochemistry Authors: Sado Y, Inoue S, Tomono Y, Matsuyama M, Fukushima M, Oohashi T, Jogahara T, Oda SI Tags: Acta Histochem Cytochem Source Type: research

In Situ Evaluation of Estrogen Receptor Dimers in Breast Carcinoma Cells: Visualization of Protein-Protein Interactions.
Abstract The estrogen receptor (ER) functions as a dimer and is involved in several different biological functions. However ER dimeric proteins have not been identified by in situ methodologies. Structured illumination microscopy (SIM) has been recently developed, which enabled the localization of protein and protein interaction. Therefore, in this study, we firstly demonstrated that ERs formed both homodimers and heterodimers in breast carcinoma cell lines using Nikon's SIM (N-SIM). ERα/α homodimers were detected in the nuclei of both ERα-positive MCF-7 and T-47D cells; 23.0% and 13.4% of ER&alp...
Source: Acta Histochemica et Cytochemica - April 27, 2017 Category: Biochemistry Authors: Iwabuchi E, Miki Y, Ono K, Onodera Y, Sasano H Tags: Acta Histochem Cytochem Source Type: research

Profiles of Periglomerular Cells in the Olfactory Bulb of Prokineticin Type 2 Receptor-deficient Mice.
Abstract Both prokineticin receptor 2 (pkr2) and prokineticin 2 (pk2) gene-deficient mice have hypoplasia of the main olfactory bulb (MOB). This hypoplasia has been attributed to disruption of the glomerulus that is caused by loss of afferent projection from olfactory sensory neurons (OSN), and to the impaired migration of granule cells, a type of interneuron. In the present study, we examined whether migration of the second type of interneuron, periglomerular cells (PGC), is dependent on the pkr2 expression by observing the localization of distinct subpopulations of PGC: calretinin (CR)-, calbindin (CB)- and tyro...
Source: Acta Histochemica et Cytochemica - April 27, 2017 Category: Biochemistry Authors: Kubo A, Sujino M, Masumoto KH, Fujioka A, Terashima T, Shigeyoshi Y, Nagano M Tags: Acta Histochem Cytochem Source Type: research

Significance of Stat3 Signaling in Epithelial Cell Differentiation of Fetal Mouse Lungs.
Abstract To study the significance of signal transducer and activator of transcription (Stat) 3 in lung epithelial development of fetal mice, we examined fetal mouse lungs, focusing on the expression of Clara cell secretory protein (CCSP), Forkhead box protein J1 (Foxj1), calcitonin gene-related peptide (CGRP), phosphorylated Stat3 (Tyr705), and hairy/enhancer of split (Hes) 1, and observed cultured fetal lungs upon treatment with IL-6, a Stat3 activator, or cucurbitacin I, a Stat3 inhibitor. Moreover, the interaction of Stat3 signaling and Hes1 was studied using Hes1 gene-deficient mice. Phosphorylated Stat3 was ...
Source: Acta Histochemica et Cytochemica - February 28, 2017 Category: Biochemistry Authors: Kameyama H, Kudoh S, Hatakeyama J, Matuo A, Ito T Tags: Acta Histochem Cytochem Source Type: research

Histological and Immunohistochemical Basis of the Effect of Aminoguanidine on Renal Changes Associated with Hemorrhagic Shock in a Rat Model.
Abstract Acute kidney failure is the main cause of death among patients with severe trauma due to massive blood loss and hemorrhagic shock (HS). Renal cell injury is caused by tissue ischemia. Renal ischemia initiates a complex and interconnected chain of events resulting in cell injury and renal cell necrosis. Nitric oxide plays a crucial role in renal function and can be inhibited by aminoguanidine (AG). We studied whether AG can ameliorate pathological renal changes associated with HS syndrome in a rat model and explored the AG protection mechanism. Rats were intraperitoneally injected with heparin sodium and m...
Source: Acta Histochemica et Cytochemica - February 28, 2017 Category: Biochemistry Authors: Al Drees A, Salah Khalil M, Soliman M Tags: Acta Histochem Cytochem Source Type: research