High-quality Fluorescence Imaging of the Human Acrosyringium Using a Transparency: Enhancing Technique and an Improved, Fluorescent Solvatochromic Pyrene Probe.
Abstract Two-photon, excitation fluorescent microscopy featuring autofluorescence or immunofluorescence, combined with optical clearance using a transparency-enhancing technique, allows deep imaging of three-dimensional (3D) skin structures. However, it remains difficult to obtain high-quality images of individual cells or 3D structures. We combined a new dye with a transparency-enhancing technology and performed high-quality structural analysis of human epidermal structures, especially the acrosyringium. Human fingertip skin samples were collected, formalin-fixed, embedded in both frozen and paraffin blocks, slic...
Source: Acta Histochemica et Cytochemica - December 25, 2020 Category: Biochemistry Authors: Murakami M, Kawakami R, Niko Y, Tsuda T, Mori H, Yatsuzuka K, Imamura T, Sayama K Tags: Acta Histochem Cytochem Source Type: research

Minocycline Alleviates Cluster Formation of Activated Microglia and Age-dependent Dopaminergic Cell Death in the Substantia Nigra of Zitter Mutant Rat.
Abstract Microglial activation is a component of neurodegenerative pathology. Here, we examine whether activated microglia participate in age-related dopaminergic (DA) cell death in the substantia nigra pars compacta (SNc) of the zitter (zi/zi) rat, a mutant characterized by deletion of the attractin gene. Confocal microscopy with double-immunohistochemical staining revealed activated microglia-formed cell-clusters surrounding DA neurons in the SNc from 2 weeks after birth. An immunoelectron microscopic study showed that the cytoplasm of activated microglia usually contains phagosome-like vacuoles and lamellar inc...
Source: Acta Histochemica et Cytochemica - December 25, 2020 Category: Biochemistry Authors: Taguchi D, Ehara A, Kadowaki T, Sakakibara SI, Nakadate K, Hirata K, Ueda S Tags: Acta Histochem Cytochem Source Type: research

Mercury and Selenium Localization in the Cerebrum, Cerebellum, Liver, and Kidney of a Minamata Disease Case.
Abstract Minamata disease is a methylmercury poisoning caused by consumption of marine food contaminated by man-made methylmercury environmental pollution, and its most prominent feature is marked pathological changes in the central nervous system. Morphological alterations are less pronounced in the liver and the kidney, although their mercury levels are higher than those of the brain. In marine mammals, methylmercury is known to be easily converted to inorganic mercury and it combines with selenium forming mercury selenide, which may counteract the toxicity of mercury. However, little is known about the formatio...
Source: Acta Histochemica et Cytochemica - December 25, 2020 Category: Biochemistry Authors: Marumoto M, Sakamoto M, Marumoto K, Tsuruta S, Komohara Y Tags: Acta Histochem Cytochem Source Type: research

Morphological and Functional Changes in Skin of Adult Male Rats Chronically Treated with Letrozole, a Nonsteroidal Inhibitor of Cytochrome P450 Aromatase.
This study indicates that estrogens exert an effect via ERs that has a role in maintaining proper skin morphology in males, together with androgen. This is also the first documented expression of FSHR in the skin of male rats. PMID: 33177782 [PubMed] (Source: Acta Histochemica et Cytochemica)
Source: Acta Histochemica et Cytochemica - October 29, 2020 Category: Biochemistry Authors: Misiakiewicz-Has K, Zawiślak A, Pilutin A, Kolasa-Wołosiuk A, Szumilas P, Duchnik E, Wiszniewska B Tags: Acta Histochem Cytochem Source Type: research

Loss of Annexin A10 Expression Is Associated with Poor Prognosis in Early Gastric Cancer.
Abstract Gastric cancer (GC) is the third most common cause of cancer-related mortality. The diagnosis and treatment of early GC is a crucial strategy for prognostic improvement of GC. Annexin A10 (ANXA10), a calcium-/phospholipid-binding protein, is a member of the annexin family. The significance of ANXA10 expression in early GC remains unclear. This is the first report to investigate ANXA10 expression in early GC. We performed immunohistochemistry to evaluate ANXA10 expression in early GC, and the correlation between ANXA10 and clinicopathological factors. The loss of ANXA10 expression was detected in 63 (61.2%...
Source: Acta Histochemica et Cytochemica - October 29, 2020 Category: Biochemistry Authors: Ishikawa A, Kuraoka K, Zaitsu J, Saito A, Kuwai T, Suzuki T, Tashiro H, Taniyama K, Yasui W Tags: Acta Histochem Cytochem Source Type: research

The Chemokine CXCL14-like Immunoreactivity Co-exists with Somatostatin, but not NPY in the Rat Dorsal Horn and Has Intimate Association with GABAergic Neurons in the Lateral Spinal Nucleus.
In this study, we investigated the distribution of CXCL14 immunoreactive structures in the rat spinal cord and clarified the association of these structures with somatostatin, glutamic acid decarboxylase (GAD; a marker for GABAergic neurons), and neuropeptide Y (NPY). CXCL14 immunoreactive fibers and puncta were observed in lamina II, which modulates somatosensation including nociception, and the lateral spinal nucleus of the spinal dorsal horn at cervical, thoracic, and lumber spinal cord levels. These CXCL14 immunoreactive structures were also immuno-positive for somatostatin, but were immuno-negative for GAD and NPY. In...
Source: Acta Histochemica et Cytochemica - October 29, 2020 Category: Biochemistry Authors: Yamamoto T, Sasaguri K, Mizumoto N, Suzuki H Tags: Acta Histochem Cytochem Source Type: research

Photodynamic Therapy Using a Novel Phosphorus Tetraphenylporphyrin Induces an Anticancer Effect via Bax/Bcl-xL-related Mitochondrial Apoptosis in Biliary Cancer Cells.
Abstract Photodynamic therapy (PDT) uses photosensitizer activation by light of a specific wavelength, and is a promising treatment for various cancers; however, the detailed mechanism of PDT remains unclear. Therefore, we investigated the anticancer effect of PDT using a novel phosphorus tetraphenylporphyrin (Ptpp) in combination with light emitting diodes (Ptpp-PDT) in the NOZ human biliary cancer cell line. Cell viability and apoptosis were examined by MTT assay, flow cytometry and TUNEL assay for 24 hr after Ptpp-PDT. MitoTracker and JC-1 were used as markers of mitochondrial localization and membrane potentia...
Source: Acta Histochemica et Cytochemica - August 25, 2020 Category: Biochemistry Authors: Mai NNH, Yamaguchi Y, Choijookhuu N, Matsumoto J, Nanashima A, Takagi H, Sato K, Tuan LQ, Hishikawa Y Tags: Acta Histochem Cytochem Source Type: research

Fatty Acid Binding Protein 7 is Involved in the Proliferation of Reactive Astrocytes, but not in Cell Migration and Polarity.
Abstract Reactive gliosis is a defense mechanism to minimize and repair the initial damage after CNS injuries that is characterized by increases in astrocytic reactivity and proliferation, with enhanced expression of glial fibrillary acidic protein (GFAP) and cellular hypertrophy. Fatty acid binding protein 7 (FABP7) is abundantly expressed in several types of glial cells, such as astrocytes and oligodendrocyte precursor cells, during brain development and FABP7-positive astrocytes have been shown to be significantly increased in the mouse cortex after a stab injury. However, the functional significance of FABP7 i...
Source: Acta Histochemica et Cytochemica - August 25, 2020 Category: Biochemistry Authors: Hara T, Abdulaziz Umaru B, Sharifi K, Yoshikawa T, Owada Y, Kagawa Y Tags: Acta Histochem Cytochem Source Type: research

Microhemorrhage in a Rat Model of Neonatal Shaking Brain Injury: Correlation between MRI and Iron Histochemistry.
Abstract Previous studies have shown that neonatal shaking brain injury (SBI) causes transient microhemorrhages (MHs) in the gray matter of the cerebral cortex and hippocampus. Iron deposits and iron-uptake cells are observed surrounding MHs in this SBI model, suggesting local hypoxic-ischemic conditions. However, whether the shaken pups suffered systemic hypoxic-ischemic conditions has remained uncertain. Further, histopathological correlations of MHs on magnetic resonance imaging (MRI) are still unclear. The present study examined MHs after neonatal SBI using a combination of histochemical and susceptibility-wei...
Source: Acta Histochemica et Cytochemica - August 25, 2020 Category: Biochemistry Authors: Taguchi D, Ehara A, Seo Y, Ueda S Tags: Acta Histochem Cytochem Source Type: research

Rab35 Targeting to the Plasma Membrane Is Dependent on the C-terminal Polybasic Cluster.
Abstract Rab35, a member of the Rab GTPase family, has been implicated in various cellular processes including cell motility and membrane trafficking. Although Rab35 is localized to the plasma membrane, Rab proteins that are identified to have high sequence homology with Rab35 exhibit distinct subcellular localization patterns. Comparing the amino acid sequences between Rab35 and its family members revealed a significant variation in an approximate 30-amino acid region of the C-terminus. This suggests that this region determines the subcellular localization of individual Rab proteins. To confirm this hypothesis, w...
Source: Acta Histochemica et Cytochemica - August 25, 2020 Category: Biochemistry Authors: Kawai K, Egami Y, Nishigaki A, Araki N Tags: Acta Histochem Cytochem Source Type: research

Vitamin D and the Host-Gut Microbiome: A Brief Overview.
Abstract There is a growing body of evidence for the effects of vitamin D on intestinal host-microbiome interactions related to gut dysbiosis and bowel inflammation. This brief review highlights the potential links between vitamin D and gut health, emphasizing the role of vitamin D in microbiological and immunological mechanisms of inflammatory bowel diseases. A comprehensive literature search was carried out in PubMed and Google Scholar using combinations of keywords "vitamin D," "intestines," "gut microflora," "bowel inflammation". Only articles published in English and re...
Source: Acta Histochemica et Cytochemica - June 25, 2020 Category: Biochemistry Authors: Akimbekov NS, Digel I, Sherelkhan DK, Lutfor AB, Razzaque MS Tags: Acta Histochem Cytochem Source Type: research

Differential Localization and Invasion of Tumor Cells in Mouse Models of Human and Murine Leukemias.
In this study, we histologically examined the distribution of leukemia cells in several organs using two leukemic mouse models produced by the administration of two cell lines (THP-1, a human myelomonocytic leukemia, and A20, a mouse B cell leukemia/lymphoma) to severe immunodeficient mice. Survival of the mice depended on the tumor burden. Although A20 and THP-1 tumor cells massively infiltrated the parenchyma of the liver and spleen at 21 days after transplantation, A20 cells were hardly found in connective tissues in Glisson's capsule in the liver as compared with THP-1 cells. In the bone marrow, there was more severe i...
Source: Acta Histochemica et Cytochemica - June 25, 2020 Category: Biochemistry Authors: Mashima K, Azuma M, Fujiwara K, Inagaki T, Oh I, Ikeda T, Umino K, Nakano H, Morita K, Sato K, Minakata D, Yamasaki R, Ashizawa M, Yamamoto C, Fujiwara SI, Hatano K, Ohmine K, Muroi K, Ohno N, Kanda Y Tags: Acta Histochem Cytochem Source Type: research

Immunohistochemical Pharmacokinetics of the Anti-diabetes Drug Alogliptin in Rat Kidney and Liver.
Abstract Alogliptin is one of a new class of therapeutic agents for type 2 diabetes called dipeptidyl peptidase-4 inhibitors. Here, we used immunohistochemistry to investigate the pharmacokinetics of alogliptin at the cell and tissue levels in the rat kidney and liver. One hour after alogliptin administration, the most noticeable immunoreactivity in the kidney was a moderate-to-strong staining in proximal tubule S3 segment epithelial cells. On the other hand, immunostaining was found only in the microvilli of S1 and S2 segment cells. Immunoreactivity was also observed in the glomerulus and distal tubules. Positive...
Source: Acta Histochemica et Cytochemica - June 25, 2020 Category: Biochemistry Authors: Yamamoto Y, Yamamoto Y, Saita T, Hira D, Chijiwa T, Shin M Tags: Acta Histochem Cytochem Source Type: research

Investigation of Novel c-Kit-expressing Smooth Muscle Cells in Murine Cecum.
Abstract In the gastrointestinal tract musculatures, c-Kit receptor tyrosine kinase is specifically expressed in interstitial cells of Cajal (ICC). ICC are distributed among the smooth muscle cells and are either bipolar or multipolar in shape. Our previous and current study shows that c-Kit-immunopositive smooth muscle cells are present in the murine cecum. Here, we found that c-Kit-expressing smooth muscle cells (named Kit-SM cells) are situated at the submucosal surface of the circular muscle layer. These cells showed smooth muscle actin and myosin immunoreactivities and ultrastructural features such as thick a...
Source: Acta Histochemica et Cytochemica - April 27, 2020 Category: Biochemistry Authors: Iino S, Horiguchi K, Horiguchi S Tags: Acta Histochem Cytochem Source Type: research

New Insights into the Pathogenesis of Diabetic Nephropathy: Proximal Renal Tubules Are Primary Target of Oxidative Stress in Diabetic Kidney.
In this study, we conducted pathohistological analyses focusing on renal tubular lesions in the early-stage diabetic kidney with the use of a streptozotocin (STZ)-induced diabetes mellitus mouse model. The results revealed that histological alterations in renal tubules, shown by a vacuolar nucleic structure, accumulations of PAS-positive substance, and accelerated restoration stress, occur initially without the presence of glomerular lesions in the early-stage diabetic kidney, and that these tubular defects are localized mainly in proximal renal tubules. Moreover, enhanced expression of RAGE, suggesting an aberrant activat...
Source: Acta Histochemica et Cytochemica - April 27, 2020 Category: Biochemistry Authors: Haraguchi R, Kohara Y, Matsubayashi K, Kitazawa R, Kitazawa S Tags: Acta Histochem Cytochem Source Type: research

Codependency of Metabolism and Epigenetics Drives Cancer Progression: A Review.
Abstract Cancer is widely considered to be a set of genetic diseases that are currently classified by tissue and cell type of origin and, increasingly, by its molecular characteristics. This latter aspect is based primarily upon oncogene gains, tumor suppressor losses, and associated transcriptional profiles. However, cancers are also characterized by profound alterations in cellular metabolism and epigenetic landscape. It is particularly noteworthy that cancer-causing genomic defects not only activate cell cycle progression, but regulate the opportunistic uptake and utilization of nutrients, effectively enabling ...
Source: Acta Histochemica et Cytochemica - February 27, 2020 Category: Biochemistry Authors: Masui K, Harachi M, K Cavenee W, S Mischel P, Shibata N Tags: Acta Histochem Cytochem Source Type: research

Incremental Growth Lines in Mouse Molar Dentin Represent 8-hr Ultradian Rhythm.
Abstract Rhythmic incremental growth lines occur in dental hard tissues of vertebrates, and dentinogenesis in rodent incisors is suggested to be controlled by the 24-hr circadian clock. Rodent incisors continue to grow throughout the animal's life; however, similar to human teeth, rodent molars stop growing after crown formation. This similarity suggests that the mouse molar is an excellent model to understand the molecular mechanisms underlying growth of human teeth. However, not much is known about the rhythmic dentinogenesis in mouse molars. Here, we investigated the incremental growth lines in mouse molar dent...
Source: Acta Histochemica et Cytochemica - December 26, 2019 Category: Biochemistry Authors: Ono R, Koike N, Inokawa H, Tsuchiya Y, Umemura Y, Yamamoto T, Kanamura N, Yagita K Tags: Acta Histochem Cytochem Source Type: research

Localization and Accumulation Studies of Dacomitinib in Rat Intestines and Skin by Immunohistochemistry.
This report is the first to elucidate the localization and accumulation of dacomitinib in the rat intestine and skin and should be valuable during efforts to clarify the mechanism dacomitinib-induced diarrhea or skin toxicities. PMID: 32001948 [PubMed] (Source: Acta Histochemica et Cytochemica)
Source: Acta Histochemica et Cytochemica - December 26, 2019 Category: Biochemistry Authors: Yamamoto Y, Saita T, Oka A, Kataoka H, Shin M Tags: Acta Histochem Cytochem Source Type: research

Hypothalamic Kisspeptin Expression in Hyperandrogenic Female Rats and Aging Rats.
Abstract Hypothalamic kisspeptin neurons stimulate gonadotropin-releasing hormone (GnRH) and luteinizing hormone (LH) release. Kisspeptin neurons in the anteroventral periventricular nucleus (AVPV) of rats induce an LH surge for ovulation, and those in the arcuate nucleus (ARC) regulate pulsatile LH secretion for follicle development and spermatogenesis. Dysfunction of kisspeptin neurons thus reduces the reproductive function. This review focuses on the effect of androgen or aging on kisspeptin expression in rats. Although androgen directly suppresses ARC kisspeptin neurons in female rats, the AVPV kisspeptin neur...
Source: Acta Histochemica et Cytochemica - October 28, 2019 Category: Biochemistry Authors: Iwata K, Kunimura Y, Ozawa H Tags: Acta Histochem Cytochem Source Type: research

Reversible Conversion among Subtypes of Salivary Gland Duct Cells as Identified by Production of a Variety of Bioactive Polypeptides.
Abstract Four major kallikreins (mK1, mK22, mK9, and mK13) were identified in the mouse submandibular gland (SMG). mK1, a true tissue kallikrein, was used as a protein marker to identify different types of SMG granular convoluted tubule (GCT) cells along with epidermal growth factor (EGF), nerve growth factor (NGF), and renin. Kallikrein mK1 was localized in a very small number (~5%) of GCT cells, which were scattered throughout the GCT, indicating that the majority of GCT cells are mK1-negative. Among mK1-positive cells, particularly strong signals were observed in a small number of narrow cells, recognized as sl...
Source: Acta Histochemica et Cytochemica - August 29, 2019 Category: Biochemistry Authors: Kurabuchi S, Yao C, Chen G, Hosoi K Tags: Acta Histochem Cytochem Source Type: research

The Effect of Estrogen on Hepatic Fat Accumulation during Early Phase of Liver Regeneration after Partial Hepatectomy in Rats.
In conclusion, these results indicated that estrogen and ERα might play an important role in fat accumulation related to FAT/CD36 during early phase of rat liver regeneration. PMID: 31592200 [PubMed] (Source: Acta Histochemica et Cytochemica)
Source: Acta Histochemica et Cytochemica - August 29, 2019 Category: Biochemistry Authors: Srisowanna N, Choijookhuu N, Yano K, Batmunkh B, Ikenoue M, Nhat Huynh Mai N, Yamaguchi Y, Hishikawa Y Tags: Acta Histochem Cytochem Source Type: research

Modulation of αvβ3 Integrin via Transactivation of β3 Integrin Gene on Murine Bone Marrow Macrophages by 1,25(OH)2D3, Retinoic Acid and Interleukin-4.
Modulation of αvβ3 Integrin via Transactivation of β3 Integrin Gene on Murine Bone Marrow Macrophages by 1,25(OH)2D3, Retinoic Acid and Interleukin-4. Acta Histochem Cytochem. 2019 Aug 30;52(4):77-83 Authors: Kitazawa S, Haraguchi R, Kohara Y, Kitazawa R Abstract The interleukin (IL)-4, 1,25(OH)2D3 and retinoic acid, increase surface expression of functional integrin αvβ3 on murine osteoclast precursors. All three agonists stimulate transcription of the β3 gene, leading to increased steady-state levels of mRNA this protein. By contrast, mRNA levels of αv remain un...
Source: Acta Histochemica et Cytochemica - August 29, 2019 Category: Biochemistry Authors: Kitazawa S, Haraguchi R, Kohara Y, Kitazawa R Tags: Acta Histochem Cytochem Source Type: research

Long-term Pilocarpine Treatment Improves Salivary Flow in Irradiated Mice.
Abstract Radiation therapy for head and neck cancer frequently causes salivary gland dysfunction. Pilocarpine is a clinically approved and effective drug that induces saliva secretion, thereby keeping the oral mucosa moist and reducing discomfort in patients, but the effect is transient. We expected that this drug also has beneficial long-term effects that maintain the integrity of salivary glands by reducing, for instance, apoptosis. Here, we examined the effects of long-term pilocarpine administration in irradiated mice. The results indicated that long-term pilocarpine administration significantly improved saliv...
Source: Acta Histochemica et Cytochemica - June 24, 2019 Category: Biochemistry Authors: Taniguchi A, Susa T, Kogo H, Iizuka-Kogo A, Yokoo S, Matsuzaki T Tags: Acta Histochem Cytochem Source Type: research

Immunohistochemical Localization of RNase 7 in Normal and Inflamed Oral Epithelia and Salivary Glands.
Abstract RNase 7 is a skin-derived antimicrobial peptide expressed in various epithelial tissues. It is upregulated by stimulation with microbes and pro-inflammatory cytokines. Herein, we examined the expression levels of RNase 7 in tissues from normal and inflamed oral epithelia and salivary glands via immunohistochemistry. RNase 7 was expressed mainly in the surface layers of the parakeratinized and orthokeratinized oral epithelium. In addition, it was strongly and weakly expressed in oral lichen planus and radicular cysts, respectively. RNase 7 was constitutively expressed in salivary glands, particularly in th...
Source: Acta Histochemica et Cytochemica - April 24, 2019 Category: Biochemistry Authors: Neopane P, Paudel D, Yoshida K, Raj Adhikari B, Morikawa T, Onishi A, Hiraki D, Uehara O, Sato J, Nishimura M, Chiba I, Abiko Y Tags: Acta Histochem Cytochem Source Type: research

The HDAC Inhibitor, SAHA, Combined with Cisplatin Synergistically Induces Apoptosis in Alpha-fetoprotein-producing Hepatoid Adenocarcinoma Cells.
In this study, we investigated the anticancer effect of SAHA in combination with cisplatin in VAT-39 cells, a newly established HAC cell line. Cell viability and apoptosis were examined by MTT assay, flow cytometry and TUNEL assay. Expression of H3S10, cleaved caspase-3, Bax, and Bcl-2 were evaluated by immunohistochemistry and western blotting. AFP levels were examined in VAT-39 cells and culture medium. Combined treatment with cisplatin and SAHA efficiently inhibited cell proliferation and decreased cell viability. Apoptotic cells, but not necrotic cells, were significantly increased following the combined treatment, and...
Source: Acta Histochemica et Cytochemica - February 27, 2019 Category: Biochemistry Authors: Kyaw MTH, Yamaguchi Y, Choijookhuu N, Yano K, Takagi H, Takahashi N, Synn Oo P, Sato K, Hishikawa Y Tags: Acta Histochem Cytochem Source Type: research

Immunohistochemical Mapping of Bcl9 Using Two Antibodies that Recognize Different Epitopes Is Useful to Characterize Juvenile Development of Hepatocellular Carcinoma in Myanmar.
In this study, we examined the expression pattern of Bcl9 immunohistochemically, using two anti-Bcl9 antibodies; one was a conventional polyclonal-antibody (anti-Bcl9ABC) against amino acid no.800-900 of human-Bcl9, while the other (anti-Bcl9BIO) was against amino acid no.50-200, covering Pygopus-binding sites of Bcl9. Immunohistochemistry using anti-Bcl9BIO demonstrated distinctive staining in the cytoplasm, while the anti-Bcl9ABC signal was detected in both cytoplasm and nuclei of HCC cells, reflecting different states of Bcl9 function because Pygopus-binding to Bcl9 is essential to exert its function together with &beta...
Source: Acta Histochemica et Cytochemica - February 27, 2019 Category: Biochemistry Authors: Soe MT, Shibata Y, Win Htun M, Abe K, Soe K, Win Than N, Lwin T, Phone Kyaw M, Koji T Tags: Acta Histochem Cytochem Source Type: research

The Role of the Wnt Signaling Pathway in Upper Jaw Development of Chick Embryo.
Abstract Cleft lip with or without cleft palate (CLP) usually results from a failure of the medial nasal prominences to fuse with the lateral and maxillary prominences. This failure inhibits facial morphogenesis regulated by several major morphogenetic signaling pathways. We hypothesized that CLP results from the failure of the Wnt signaling pathway. To examine whether Wnt signaling can influences upper jaw development, we applied beads soaked with Dickkopf-1 (Dkk-1), Alsterpaullone (AL) or Wnt3a to the right side of the maxillary prominence of the chick embryo. The embryo showed a defect of the maxilla on the tre...
Source: Acta Histochemica et Cytochemica - February 27, 2019 Category: Biochemistry Authors: Shimomura T, Kawakami M, Tatsumi K, Tanaka T, Morita-Takemura S, Kirita T, Wanaka A Tags: Acta Histochem Cytochem Source Type: research

Immunohistochemistry for Anti-diabetes Drug, Alogliptin Using a Newly Prepared Monoclonal Antibody: Its Precise Localization in Rat Small Intestine.
Abstract Knowledge of time sequence of localization of drugs in cells and tissues of animals may help in developing a better understanding of the actual overall pharmacokinetics of the drugs. We produced monoclonal antibody (mAb) against alogliptin (AG), a dipeptidyl peptidase-4 (DPP-4) inhibitor, conjugated to BSA with N-(γ-maleimidobutyryloxy)-succinimide. The mAb was specific for AG and did not cross-react with sitagliptin, vancomycin or amoxicillin. The mAb enabled us to develop an immunohistochemical method for detecting the localization of AG in the rat small intestine. One hour after a single oral adm...
Source: Acta Histochemica et Cytochemica - February 27, 2019 Category: Biochemistry Authors: Yamamoto Y, Yamamoto Y, Saita T, Shin M Tags: Acta Histochem Cytochem Source Type: research

New Insight into the Role of Nitric Oxide Pathways in Pancreas.
Abstract Nitric oxide (NO) is generated by a family of enzymes termed NO synthases (NOS) that convert L-arginine to NO and citrulline. The role of NO as an important biological mediator and recognition of the pathophysiological significance of superoxides/NO interaction has led to an intensive research and development of therapies based on the interception of the NO signaling cascade in the pancreatitis course. However, the presence and localization of the NO-generating enzymes in various organs including pancreas are subject to controversy. We assumed that this controversy might reflect rather the diversity of ex...
Source: Acta Histochemica et Cytochemica - December 20, 2018 Category: Biochemistry Authors: Buchwalow I, Schnekenburger J, Samoilova V, Boecker W, Neumann J, Tiemann K Tags: Acta Histochem Cytochem Source Type: research

Stem-like Human Breast Cancer Cells Initiate Vasculogenic Mimicry on Matrigel.
Abstract Vasculogenic mimicry (VM), referring to vasculogenic structures lined by tumor cells, can be distinguished from angiogenesis, and is responsible for the aggressiveness and metastatic potential of tumors. HCC1937/p53 cells were derived from triple-negative breast cancer (TNBC), and used to investigate the roles of breast cancer stem cells (CSCs) in the formation of VM. HCC1937/p53 cells formed mesh-like structures on matrigel culture in which expression of VM-related genes, vascular endothelial (VE)-cadherin, matrix metalloproteinase (MMP)-2 and MMP-9 was confirmed by droplet digital polymerase chain react...
Source: Acta Histochemica et Cytochemica - December 20, 2018 Category: Biochemistry Authors: Izawa Y, Kashii-Magaribuchi K, Yoshida K, Nosaka M, Tsuji N, Yamamoto A, Kuroyanagi K, Tono K, Tanihata M, Imanishi M, Onishi M, Sakiyama M, Inoue S, Takahashi R Tags: Acta Histochem Cytochem Source Type: research

Collapsin Response Mediator Protein 1, a Novel Marker Protein for Differentiated Odontoblasts.
Abstract We previously reported that the terminal differentiation of odontoblasts was inhibited in Runx2 transgenic {Tg(Col1a1-Runx2)} mice under the control of the 2.3-kb Col1a1 promoter. Odontoblasts in Tg(Col1a1-Runx2) mice lose their characteristic long cellular processes, and show marked reductions in the protein levels of markers for odontoblasts, such as dentin sialophosphoprotein, nestin, and microtubule-associated protein tau (Mapt). We herein demonstrated that collapsin response mediator protein 1 (CRMP1), a neuronal phosphoprotein that participates in various aspects of neuronal development, was specifi...
Source: Acta Histochemica et Cytochemica - December 20, 2018 Category: Biochemistry Authors: Miyazaki T, T Baba T, Mori M, Komori T Tags: Acta Histochem Cytochem Source Type: research

Development of Chemical Tools for Imaging of Fe(II) Ions in Living Cells: A Review.
Abstract Intracellular labile iron is an iron species which is not or weakly bound to proteins and depicts an important effect on homeostatic regulation in cells. An excess or deficiency of iron can cause oxidative damage to key cellular biomolecules. The behavior and concentrations of labile iron are difficult to monitor, but the specific redox state of the Fe ions is relevant to the physiological and pathological properties that we would like to study. We have developed a series of turn-on type fluorescent probes that are highly selective to the labile Fe(II) ions, and we have tested their applications to cellul...
Source: Acta Histochemica et Cytochemica - October 31, 2018 Category: Biochemistry Authors: Hirayama T Tags: Acta Histochem Cytochem Source Type: research

Immunohistochemical Study of the Laminin α5 Chain and Its Specific Receptor, Basal Cell Adhesion Molecule (BCAM), in both Fetal and Adult Rat Pituitary Glands.
Immunohistochemical Study of the Laminin α5 Chain and Its Specific Receptor, Basal Cell Adhesion Molecule (BCAM), in both Fetal and Adult Rat Pituitary Glands. Acta Histochem Cytochem. 2018 Oct 31;51(5):145-152 Authors: Azuma M, Tsukada T, Inagaki T, Casmad F, Jindatip D, Tofrizal A, Maliza R, Batchuluun K, Syaidah R, Ohno N, Fujiwara K, Kikuchi M, Yashiro T Abstract Laminin, a major basement membrane protein, comprises three subunit chains: α, β, and γ chains. Among these chains, only the laminin α chain is capable of signaling via laminin receptors. Although laminin isof...
Source: Acta Histochemica et Cytochemica - October 31, 2018 Category: Biochemistry Authors: Azuma M, Tsukada T, Inagaki T, Casmad F, Jindatip D, Tofrizal A, Maliza R, Batchuluun K, Syaidah R, Ohno N, Fujiwara K, Kikuchi M, Yashiro T Tags: Acta Histochem Cytochem Source Type: research

Immunocytochemical Localization of Choline Acetyltransferase in the Microbat Visual Cortex.
Abstract The purpose of the present study was to investigate the organization of choline acetyltransferase (ChAT)-immunoreactive (IR) fibers in the visual cortex of the microbat, using standard immunocytochemistry and confocal microscopy. ChAT-IR fibers were distributed throughout all layers of the visual cortex, with the highest density in layer III and the lowest density in layer I. However, no ChAT-IR cells were found in the microbat visual cortex. ChAT-IR fibers were classified into two types: small and large varicose fibers. Previously identified sources of cholinergic fibers in the mammalian visual cortex, t...
Source: Acta Histochemica et Cytochemica - October 31, 2018 Category: Biochemistry Authors: Kim GH, Kim HG, Jeon CJ Tags: Acta Histochem Cytochem Source Type: research

Intranuclear Mobility of Estrogen Receptor: Implication for Transcriptional Regulation.
Abstract The estrogen receptor (ER) is a ligand-dependent transcription factor that has two subtypes: ERα and ERβ. ERs regulate transcription of estrogen-responsive genes through interactions with multiple intranuclear components, such as cofactors and the nuclear matrix. Live cell imaging using fluorescent protein-labeled ERs has revealed that ligand-activated ERs are highly mobile in the nucleus, with transient association with the DNA and nuclear matrix. Scaffold attachment factor B (SAFB) 1 and its paralogue, SAFB2, are nuclear matrix-binding proteins that negatively modulate ERα-mediated tran...
Source: Acta Histochemica et Cytochemica - August 29, 2018 Category: Biochemistry Authors: Matsuda KI, Hashimoto T, Kawata M Tags: Acta Histochem Cytochem Source Type: research

Label-free Molecular Imaging and Analysis by Raman Spectroscopy.
Abstract Raman scattering of a cell conveys the intrinsic information inherent to chemical structures of biomolecules. The spectroscopy of Raman scattering, or Raman spectroscopy, allows label-free and quantitative molecular sensing of a biological sample in situ without disruption. For the last five decades Raman spectroscopy has been widely utilized in biological research fields. However, it is just within the latest decade that molecular imaging and discrimination of living cells and tissues have become practically available. Here we overview recent progress in Raman spectroscopy and its application to life sci...
Source: Acta Histochemica et Cytochemica - June 26, 2018 Category: Biochemistry Authors: Kumamoto Y, Harada Y, Takamatsu T, Tanaka H Tags: Acta Histochem Cytochem Source Type: research

TRK-fused Gene Protein Localization Is Prominent in Serotonergic and Noradrenergic Cell Groups, and Some Lower Motor Neurons in the Corticospinal Tract of the Rat Brainstem.
In this study, we employed double immunohistochemistry to investigate the details of TFG localization patterns in monoaminergic and cholinergic neurons in the brainstem. Intense TFG immunoreactivity was observed in the dorsal raphe nucleus, the locus coeruleus, and the ventral horn of the spinal cord. TFG immunoreactivity was observed in some serotonergic neurons in all B1-B9 cell groups, and some noradrenergic neurons in all A1-A7 cell groups in the rat brainstem, while no immunoreactivity was observed in the dopaminergic neurons in A8-A10 cell groups. TFG immunoreactivity was observed in all ChAT-positive motor nuclei in...
Source: Acta Histochemica et Cytochemica - June 26, 2018 Category: Biochemistry Authors: Takeuchi S, Tooyama I Tags: Acta Histochem Cytochem Source Type: research

Biochemical and Morphological Characterization of a Guanine Nucleotide Exchange Factor ARHGEF9 in Mouse Tissues.
In this study, we prepared a specific polyclonal antibody against ARHGEF9, anti-ARHGEF9, and carried out expression analyses with mouse tissues especially brain. Western blotting analyses demonstrated tissue-dependent expression profiles of ARHGEF9 in the young adult mouse, and strongly suggested a role during brain development. Immunohistochemical analyses revealed developmental stage-dependent expression profiles of ARHGEF9 in cerebral cortex, hippocampus and cerebellum. ARHGEF9 exhibited partial localization at dendritic spines in cultured hippocampal neurons. From the obtained results, anti-ARHGEF9 was found to be a us...
Source: Acta Histochemica et Cytochemica - June 26, 2018 Category: Biochemistry Authors: Ibaraki K, Mizuno M, Aoki H, Niwa A, Iwamoto I, Hara A, Tabata H, Ito H, Nagata KI Tags: Acta Histochem Cytochem Source Type: research

Electron Microscopic Study Using Formalin-fixed, Paraffin-embedded Material, with Special Reference to Observation of Microbial Organisms and Endocrine Granules.
Abstract In the diagnostic pathology practice, specimens for electron microscopy (EM) are not necessarily handled and fixed under the ideal condition. In the present article, the author describes ultrastructural study using formalin-fixed and/or paraffin-embedded material. The fine morphologic preservation is often acceptable, particularly when small cubes are dug out of paraffin blocks. Particulate structures such as neuroendocrine granules and microbes are consistently observed even using paraffin sections. Paraffin sections signalized with silver particles in Grimelius and Grocott stains or diaminobenzidine pro...
Source: Acta Histochemica et Cytochemica - April 27, 2018 Category: Biochemistry Authors: Tsutsumi Y Tags: Acta Histochem Cytochem Source Type: research

Effect of Daily Light on c-Fos Expression in the Suprachiasmatic Nucleus under Jet Lag Conditions.
Abstract Jet-lag symptoms arise from temporal misalignment between the internal circadian clock and external solar time when traveling across multiple time zones. Light is known as a strong timing cue of the circadian clock. We here examined the effect of daily light on the process of jet lag by detecting c-Fos expression in the master clock neurons in the suprachiasmatic nucleus (SCN) under 8-hr phase-advanced jet lag condition. In WT mice, c-Fos-immunoreactivity was found at 1-2 hours on the first day after light/dark (LD) phase-advance. This induction was also observed on the second and third days, although the...
Source: Acta Histochemica et Cytochemica - April 27, 2018 Category: Biochemistry Authors: Chen Y, Yamaguchi Y, Suzuki T, Doi M, Okamura H Tags: Acta Histochem Cytochem Source Type: research

Morphological Analysis of Trafficking and Processing of Anionic and Cationic Liposomes in Cultured Cells.
Abstract Liposomes, artificial phospholipid vesicles, have been developed as a non-viral drug delivery system to allow contained agents to be efficiently delivered to target sites via systemic circulation. Liposomes have been used as a gene transfer tool with cultured cells; however, their precise trafficking and processing remain uncertain. Furthermore, liposomes with different surface charges are known to exhibit distinct properties. The purpose of the current study was to elucidate the intracellular trafficking and processing of liposomes with anionic and cationic surface charges from a morphological view point...
Source: Acta Histochemica et Cytochemica - April 27, 2018 Category: Biochemistry Authors: Tomori Y, Iijima N, Hinuma S, Ishii H, Takumi K, Takai S, Ozawa H Tags: Acta Histochem Cytochem Source Type: research

Changes in DNA Methylation of Oocytes and Granulosa Cells Assessed by HELMET during Folliculogenesis in Mouse Ovary.
In this study, we examined changes in the methylation level of CCGG and GATCG sites during mouse folliculogenesis in paraffin-embedded sections of mouse ovaries. For the purpose, we used a new method, histo endonuclease-linked detection of methylation sites of DNA (HELMET), designed to detect methylation sites of DNA with a specific sequence in a tissue section. Unlike the global level of DNA methylation, which was no change in immunohistochemical staining of 5-methylcytosine throughout folliculogenesis, we found that there were hypermethylation of CCGG and GATCG sites in most of the granulosa cells of tertiary follicles c...
Source: Acta Histochemica et Cytochemica - April 27, 2018 Category: Biochemistry Authors: Liu J, Zhang W, Wu Z, Dai L, Koji T Tags: Acta Histochem Cytochem Source Type: research

A Newly Defined Area of the Mouse Anterior Hypothalamus Involved in Septohypothalamic Circuit: Perifornical Area of the Anterior Hypothalamus, PeFAH.
In this study, we identified a new triangular-shaped area between the paraventricular hypothalamic nucleus (PVN) and the fornix area in the mouse anterior hypothalamus, which is enriched in chondroitin sulfate proteoglycans (CSPGs). We designated this region as the perifornical area of the anterior hypothalamus (PeFAH) based on its anatomical location. As evidenced by Nissl staining, the PeFAH was distinguishable as an area of relatively low density. Immunohistochemical and DNA microarray analyses indicated that PeFAH contains sparsely distributed calretinin-positive neurons and densely clustered enkephalin-positive neuron...
Source: Acta Histochemica et Cytochemica - February 27, 2018 Category: Biochemistry Authors: Horii-Hayashi N, Nishi M Tags: Acta Histochem Cytochem Source Type: research

Improved Serial Sectioning Techniques for Correlative Light-Electron Microscopy Mapping of Human Langerhans Islets.
In conclusion, the correlative imaging approach developed by us may be useful for examining ultrastructural features in combination with immunolocalisation of endocrine hormones in the same human pancreatic islets. PMID: 29622846 [PubMed] (Source: Acta Histochemica et Cytochemica)
Source: Acta Histochemica et Cytochemica - February 27, 2018 Category: Biochemistry Authors: Saitoh S, Ohno N, Saitoh Y, Terada N, Shimo S, Aida K, Fujii H, Kobayashi T, Ohno S Tags: Acta Histochem Cytochem Source Type: research

Estrogen Regulates Mitochondrial Morphology through Phosphorylation of Dynamin-related Protein 1 in MCF7 Human Breast Cancer Cells.
Abstract Estrogen affects mitochondrial function in various tissues, but the precise mechanism remains unclear. We, therefore investigated the effect on estrogen-regulated mitochondrial morphology by dynamin-related protein 1 (Drp1) and its Ser616-phosphorylated derivative (pDrp1Ser616) are involved in mitochondrial fission. MCF7 human breast cancer cells were treated with 17β-estradiol (E2), an estrogen receptor (ER) α and β antagonist (ICI 182, 780), an ERα antagonist (MPP), and an ERβ antagonist (PHTPP) for 24 hr. The expression of Drp1 and pDrp1Ser616 was analyzed by western blotting...
Source: Acta Histochemica et Cytochemica - February 27, 2018 Category: Biochemistry Authors: Oo PS, Yamaguchi Y, Sawaguchi A, Tin Htwe Kyaw M, Choijookhuu N, Noor Ali M, Srisowanna N, Hino SI, Hishikawa Y Tags: Acta Histochem Cytochem Source Type: research

The HDAC Inhibitor, SAHA, Prevents Colonic Inflammation by Suppressing Pro-inflammatory Cytokines and Chemokines in DSS-induced Colitis.
Abstract Inflammatory bowel disease (IBD) is an inflammatory disorder of the gastrointestinal tract that is caused by multiple factors, including dysfunction of the immune system and genetic and epigenetic alterations. Aberrant epigenetic regulation, especially histone acetylation, was found in biopsies from IBD patients and mouse models of colitis, suggesting that an epigenetic treatment approach may be useful for IBD therapy. Therefore, we investigated the effects of the histone deacetylase (HDAC) inhibitor, suberoylanilide hydroxamic acid (SAHA), in a mouse model of dextran sulfate sodium (DSS)-induced colitis....
Source: Acta Histochemica et Cytochemica - February 27, 2018 Category: Biochemistry Authors: Ali MN, Choijookhuu N, Takagi H, Srisowanna N, Nguyen Nhat Huynh M, Yamaguchi Y, Synn Oo P, Tin Htwe Kyaw M, Sato K, Yamaguchi R, Hishikawa Y Tags: Acta Histochem Cytochem Source Type: research

Expression and Dynamics of Podoplanin in Cultured Osteoblasts with Mechanostress and Mineralization Stimulus.
This study investigates the significance of the expression and dynamics of podoplanin in mechanostress and mineralization in cultured murine osteoblasts. Podoplanin increased in osteoblasts subjected to straining in non-mineralization medium, suggesting that the mechanostress alone is a podoplanin induction factor. In osteoblasts subjected to vertical elongation straining in the mineralization medium, the mRNA amounts of podoplanin, osteopontin, and osteocalcin were significantly larger than those in cells not subjected to straining, suggesting that mechanostress is the cause of a synergistic effect in the expression of th...
Source: Acta Histochemica et Cytochemica - February 27, 2018 Category: Biochemistry Authors: Takenawa T, Kanai T, Kitamura T, Yoshimura Y, Sawa Y, Iida J Tags: Acta Histochem Cytochem Source Type: research

Expression of Draxin in Lung Carcinomas.
Abstract Guidance molecules, such as Netrin-1, and their receptors have important roles in controlling axon pathfinding, modulate biological activities of various cancer cells, and may be a useful target for cancer therapy. Dorsal repulsive axon guidance protein (Draxin) is a novel guidance molecule that binds not only common guidance molecule receptors with Netrin-1, but also directly binds the EGF domain of Netrin-1 through a 22-amino-acid peptide (22aa). By immunostaining, Draxin was positively expressed in small cell carcinoma, adenocarcinoma (ADC), and squamous cell carcinoma of the lung. In addition, western...
Source: Acta Histochemica et Cytochemica - February 27, 2018 Category: Biochemistry Authors: Sato Y, Matsuo A, Kudoh S, Fang L, Hasegawa K, Shinmyo Y, Ito T Tags: Acta Histochem Cytochem Source Type: research

CXCL14-like Immunoreactivity Exists in Somatostatin-containing Endocrine Cells, and in the Lamina Propria and Submucosal Somatostatinergic Nervous System of Mouse Alimentary Tract.
Abstract In the present study, we investigated the distribution of CXCL14 immunoreactive endocrine cells and neurons in mouse alimentary tract by immunohistochemistry. CXCL14 immunoreactive endocrine cells were found as closed-type cells in the stomach and open-type cells in the small intestine. The immunostaining of these endocrine cells corresponded with that of the somatostatin-containing endocrine cells. Only a few CXCL14 immunoreactive endocrine cells were seen in the large intestine. CXCL14 immunoreactive fibers were observed in the muscular layer from the stomach to the rectum with most abundance in the rec...
Source: Acta Histochemica et Cytochemica - December 26, 2017 Category: Biochemistry Authors: Suzuki H, Yamada K, Matsuda Y, Onozuka M, Yamamoto T Tags: Acta Histochem Cytochem Source Type: research

CCN3 Expression Marks a Sulfomucin-nonproducing Unique Subset of Colonic Goblet Cells in Mice.
Abstract Intestinal goblet cells are characterized by their unique morphology and specialized function to secrete mucins. Although it is known that they are a heterogeneous population of cells, there have been few studies that relate the expression of a particular gene with functionally distinct subpopulations of intestinal goblet cells. Here we show that CCN3, a gene encoding a member of the CCN family proteins, is induced by inhibition of Notch signaling in colonic epithelial cells and expressed in goblet cells in mice. We demonstrate that CCN3 expression is confined to a subpopulation of goblet cells in the low...
Source: Acta Histochemica et Cytochemica - December 26, 2017 Category: Biochemistry Authors: Akiyama S, Mochizuki W, Nibe Y, Matsumoto Y, Sakamoto K, Oshima S, Watanabe M, Nakamura T Tags: Acta Histochem Cytochem Source Type: research