Regulation of PDZ domain containing 1 (PDZK1) Expression by Hepatocyte Nuclear Factor 1 alpha (HNF1 α) in Human Kidney.

Regulation of PDZ domain containing 1 (PDZK1) Expression by Hepatocyte Nuclear Factor 1 alpha (HNF1α) in Human Kidney. Am J Physiol Renal Physiol. 2017 Jul 19;:ajprenal.00650.2016 Authors: Prestin K, Hussner J, Ferreira C, Seibert I, Breitung V, Zimmermann U, Meyer Zu Schwabedissen HE Abstract In the renal proximal tubule the secretion and reabsorption of glomerularly filtrated compounds is realized by a functional network of uptake and efflux transporters. The activity and localization of several transporters expressed at the apical tubular membrane is regulated by the membrane associated protein PDZ domain containing 1 (PDZK1). We aimed to characterize the transcriptional regulation of this modulator of renal transport. Coexpression analyses of PDZK1 and putative regulators were performed using human kidney samples. Protein and mRNA expression of PDZK1 in renal proximal tubule epithelial cells after adenoviral transfer and siRNA knockdown of transcription factor hepatocyte nuclear factor 1 alpha (HNF1α) was assessed by quantitative real-time PCR and Western blot analysis. Transactivation of the PDZK1 promoter was quantified in cell-based reporter gene assays. Subsequently, the binding of HNF1α to the PDZK1 promoter was verified by in silico analyses and chromatin immunoprecipitation assay. HNF1α positively regulated the promoter activity of PDZK1. Adenoviral overexpression of HNF1α in renal proximal tubule epithelial cells (RP...
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research