Molecular typing of Avian Pathogenic Escherichia coli isolates originating from outbreaks of E. coli peritonitis syndrome in chicken flocks.

Molecular typing of Avian Pathogenic Escherichia coli isolates originating from outbreaks of E. coli peritonitis syndrome in chicken flocks. Avian Pathol. 2014 Jun 19;:1-33 Authors: Landman WJ, Buter R, Dijkman R, van Eck JH Abstract Escherichia coli strains isolated from the bone marrow of fresh dead hens of laying flocks with the E. coli peritonitis syndrome (EPS) were genotyped using Pulsed-Field Gel Electrophoresis (PFGE). Mentioned typing is important from an epidemiological point of view and also if the use of (auto)vaccines is considered. Birds with EPS originated from one house of each of three layer farms and one broiler breeder farm. Farms were considered as separate epidemiological units. In total, six flocks were examined including two successive flocks of one layer farm and the broiler breeder farm. E. coli colonies (1 per bird) from 9 to 16 hens of each flock were genotyped. All colonies were assumed to be pure cultures. The clonality of E. coli within birds was studied using 5 colonies of each of 9 to 14 birds per flock. E. coli genotypes, which totalized 15, differed between farms and flocks except for two successive layer flocks that shared 3 genotypes. One to five genotypes were found per flock with one or two genotypes dominating each outbreak. Within hens E. coli bacteria were always clonal. Colonies of the same PFGE-type always had the same multilocus sequence type (MLST). However, four PFGE-types shared sequence...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research