Molecular epidemiology of Microsporum canis isolated in Japan based on multilocus microsatellite typing fragment analysis.

Molecular epidemiology of Microsporum canis isolated in Japan based on multilocus microsatellite typing fragment analysis. Jpn J Infect Dis. 2017 Jul 01;: Authors: Watanabe J, Anzawa K, Mochizuki T Abstract Intraspecies polymorphisms of Microsporum canis strains isolated from human lesions and domesticated cats in Japan were examined by multilocus microsatellite (MS) analysis. Using nine MS markers, 70 strains were classified into 20 genotypes (genotypes A to T). Of the nine MS markers, five were useful for genotyping, whereas the other four were not. The combination of MS2, MS4 and MS7 has the highest resolution power for inter-strain differentiation. Genotype A, present in 15 strains (21.4%), was the most frequent, followed by genotypes H (10 strains, 14.3%), P (eight strains, 11.4%), I (six strains, 8.6%) and S (six strains, 8.6%). Eight individual genotypes were present in one strain each. Five episodes of infection of humans and cats living in the same households were analyzed, with the strains in all five households showing the same genotypes. Analysis of their geographical distribution showed that both genotypes A and H were isolated from six prefectures on Honshu and Kyushu islands. To our knowledge, this is the first report showing intraspecies polymorphisms of M. canis isolated in Japan by molecular methods. PMID: 28674311 [PubMed - as supplied by publisher]
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research