Thermal Inactivation of avian influenza virus in poultry litter as a method to decontaminate poultry houses

The objective was to determine the time necessary to inactivate AIV in poultry litter at temperatures achievable in a poultry house. Low pathogenic (LP) AIV inactivation was evaluated between 10.0°-48.9° C, at ∼5.5° C intervals and highly pathogenic (HP) AIV inactivation was evaluated between 10.0°-43.3° C, at ∼11° C intervals. Samples were collected at numerous time points for each temperature. Virus isolation in embryonating chicken eggs was conducted to determine if viable virus was present. Each sample was also tested by real-time RT-PCR. Low pathogenicity AIV was inactivated at 1day at 26.7° C or above. At 10.0, 15.6 and 21.1° C, inactivation times increased to 2-5 days. Highly pathogenic AIV followed a similar trend; the virus was inactivated after 1day at 43.3° C and 32.2° C, and required 2 and 5days for inactivation at 21.1° C and 10.0° C respectively. While low pathogenicity AIV appeared to be inactivated at a lower temperature than high pathogenicity AIV, this was not due to any difference in the strains, but due to fewer temperature points evaluated for high pathogenicity. Endpoints for detection by real-time RT-PCR were not found even weeks after the virus was inactivated. This provides a guideline for the time required, at specific temperatures to inactivate AIV in poultry litter and likely on surfaces within the house. Heat treatment will provide an added level of safety to personnel and against further spread by eliminating infectious virus prio...
Source: Preventive Veterinary Medicine - Category: Veterinary Research Source Type: research