Crystal structure of enolase from Drosophila melanogaster

Enolase is an important enzyme in glycolysis and various biological processes. Its dysfunction is closely associated with diseases. Here, the enolase from Drosophila melanogaster (DmENO) was purified and crystallized. A crystal of DmENO diffracted to 2.0   Å resolution and belonged to space group R32. The structure was solved by molecular replacement. Like most enolases, DmENO forms a homodimer with conserved residues in the dimer interface. DmENO possesses an open conformation in this structure and contains conserved elements for catalytic activity. This work provides a structural basis for further functional and evolutionary studies of enolase.
Source: Acta Crystallographica Section F - Category: Biochemistry Authors: Tags: enolase crystal structure active site dimerization glycolysis Drosophila melanogaster research communications Source Type: research