Enzyme-mediated ligation technologies for peptides and proteins.

Enzyme-mediated ligation technologies for peptides and proteins. Curr Opin Chem Biol. 2017 Feb 18;38:1-7 Authors: Schmidt M, Toplak A, Quaedflieg PJ, Nuijens T Abstract With the steadily increasing complexity and quantity requirements for peptides in industry and academia, the efficient and site-selective ligation of peptides and proteins represents a highly desirable goal. Within this context, enzyme-mediated ligation technologies for peptides and proteins have attracted great interest in recent years as they represent an extremely powerful extension to the scope of chemical methodologies (e.g. native chemical ligation) in basic and applied research. Compared to chemical ligation methods, enzymatic strategies using ligases such as sortase, butelase, peptiligase or omniligase generally feature excellent chemoselectivity, therefore making them valuable tools for protein and peptide chemists. PMID: 28229906 [PubMed - as supplied by publisher]
Source: Current Opinion in Chemical Biology - Category: Biochemistry Authors: Tags: Curr Opin Chem Biol Source Type: research