Frequency of Th17 cells correlates with the presence of lung lesions in pigs chronically infected with Actinobacillus pleuropneumoniae

AbstractPorcine contagious pleuropneumonia caused byActinobacillus pleuropneumoniae (APP) remains one of the major causes of poor growth performance and respiratory disease in pig herds. While the role of antibodies against APP has been intensely studied, the porcine T cell response remains poorly characterized. To address this, pigs were intranasally infected with APP serotype 2 and euthanized during the acute phase [6 –10 days post-infection (dpi)] or the chronic phase of APP infection (27–31 dpi). Lymphocytes isolated from blood, tonsils, lung tissue and tracheobronchial lymph nodes were analyzed by intracellular cytokine staining (ICS) for IL-17A, IL-10 and TNF-α production after in vitro stimulation wit h crude capsular extract (CCE) of the APP inoculation strain. This was combined with cell surface staining for the expression of CD4, CD8α and TCR-γδ. Clinical records, microbiological investigations and pathological findings confirmed the induction of a subclinical APP infection. ICS-assays reve aled the presence of APP-CCE specific CD4+CD8 αdim IL-17A-producing T cells in blood and lung tissue in most infected animals during the acute and chronic phase of infection and a minor fraction of these cells co-produced TNF- α. APP-CCE specific IL-17A-producing γδ T cells could not be found and APP-CCE specific IL-10-producing CD4+ T cells were present in various organs but only in a few infected animals. The...
Source: Veterinary Research - Category: Veterinary Research Source Type: research

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Conditions:   Pain, Postoperative;   Tonsillar Bleeding;   Postoperative Nausea and Vomiting Interventions:   Drug: Dexamethasone;   Drug: Sodium chloride Sponsors:   Walid HABRE;   University Hospital, Geneva Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
AbstractPeriodic Fever, Aphthous stomatitis, Pharyngitis and Adenitis (PFAPA) syndrome is an inflammatory disorder of childhood classically characterized by recurrent fevers, pharyngitis, stomatitis, cervical adenitis, and leukocytosis. While the mechanism is unclear, previous studies have shown that tonsillectomy can be a therapeutic option with improvement in quality of life in many patients with PFAPA, but the mechanisms behind surgical success remain unknown. In addition, long-term clinical follow-up is lacking. In our tertiary care center cohort, 62 patients with PFAPA syndrome had complete resolution of symptoms afte...
Source: Journal of Clinical Immunology - Category: Allergy & Immunology Source Type: research
ConclusionsRhinovirus species associated differently with clinical characteristics and tonsillar cytokine responses.
Source: Clinical and Translational Allergy - Category: Allergy & Immunology Source Type: research
Publication date: Available online 29 November 2019Source: Journal of Pediatric Surgery Case ReportsAuthor(s): Ahmed Aldandan, Omair Al Hussain, Salman Alhajri, Ali AlmomenAbstractSince the advent of antibiotics, major spontaneous tonsillar hemorrhage is extremely rare. The majority of the spontaneous tonsillar hemorrhage events is associated with acute or chronic tonsillitis, coagulopathies, or tonsillar cancer. Here, we report a very rare case of spontaneous tonsillar hemorrhage in a 7 year old girl managed by emergency tonsillectomy and IV antibiotic with no complications.
Source: Journal of Pediatric Surgery Case Reports - Category: Surgery Source Type: research
This study was conducted among consecutive adults, who were admitted to the Department of Pulmonary Medicine, Marmara University, Istanbul between November 2015 and October 2018. Participants answered the questionnaires and underwent a sleep study with cardiorespiratory polygraphy. OSA was defined as an Apnoe-Hypopnoe-Index (AHI)≥15/h.In all, 1042 adults (334 women; 32.1%) were included. OSA was diagnosed in 589 (56.5%). In the OSA group, women were older (53.7±11.5 vs 47.8± 12.8 yrs; p
Source: European Respiratory Journal - Category: Respiratory Medicine Authors: Tags: Sleep and control of breathing Source Type: research
Conclusion: Delta 12s can be used as a simple screening test to identify children without Down’s syndrome with a high likelihood of OSA.References:1. Magalang UJ et al. Chest 2003;124:1694-7012. Hill CM et al. Arch Dis Child 2018;103:962-7
Source: European Respiratory Journal - Category: Respiratory Medicine Authors: Tags: Paediatric respiratory physiology and sleep Source Type: research
Authors: Yücel Ekici N, Külahci Ö Abstract BACKGROUND: Previous reports suggested that allergic/eosinophilic inflammation affects the adenoid and tonsillar tissue. The aim of this study is to evaluate and compare the tissue and serum eosinophilia in children undergoing adeno-tonsillectomy with allergic rhinitis. MATERIALS AND METHODS: The clinical registers of 125 children who underwent adenoidectomy/tonsillectomy due to adenoid/tonsil hypertrophy were examined and reviewed retrospectively. 57 children with skin prick test positivity and with symptoms of allergic rhinitis were included in the stu...
Source: Turkish Journal of Medical Sciences - Category: General Medicine Tags: Turk J Med Sci Source Type: research
Laryngo-Rhino-Otol 2019; 98: 760-761 DOI: 10.1055/a-0898-8562Tzelnick S et al. Long-Term Outcomes of Tonsillectomy for Recurrent Tonsillitis in Adults. Laryngoscope 2019; 9999: 1–4. doi:10.1002/lary.27928 Ein Cochrane Review von 2014 beantwortete die Frage nach der Effektivität von Tonsillektomien bei Erwachsenen nicht abschließend, sprach aber für einen positiven Einfluss auf die Lebensqualität. Die retrospektive Studie aus Israel belegt nun eine deutliche Reduktion der Tonsillitis-Episoden und der Gesundheitskosten. [...] © Georg Thieme Verlag KG Stuttgart · New YorkArticl...
Source: Laryngo- Rhino- Otologie - Category: ENT & OMF Authors: Tags: Referiert & Diskutiert Source Type: research
This study aims to explore the effects of low-temperature plasma treatment on pulmonary function in children with OSAHS.MethodsA total of 110 children with OSAHS were included in this prospective study. Low-temperature plasma radiofrequency treatment and routine surgical treatment were performed in group A and group B, respectively. Maximal voluntary ventilation (MVV), forced vital capacity (FVC), and total lung capacity (TLC) were measured. OSA-18 survey was used to evaluate the quality of life 1 year after operation.ResultsGroup A had significantly higher effective treatment rate (P
Source: Irish Journal of Medical Science - Category: General Medicine Source Type: research
Conditions:   Tonsillitis;   Postoperative Pain;   Cryotherapy Effect Intervention:   Dietary Supplement: 2 daily standard doses of vanilla-chocolate ice cream (cryotherapy) Sponsor:   University Hospital  " Sestre  Milosrdnice " Recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
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