Stereo- and region-specific biotransformation of physapubescin by four fungal strains

AbstractBiotransformations of physapubescin (1) were performed by four fungal strains —Mucor subtilissimus AS 3.2454,Mucor polymorphosporus AS 3.3443,Aspergillus niger AS 3.795, andSyncephalastrum racemosum AS 3.264. Four metabolites were prepared in the biotransformation process of1, and their structures were elucidated as 15α-acetoxy-5,6β:22,26:24,25-triepoxy-26α-hydroxy-3β-methoxy 4β-hydroxyergost-1-one (2), 15α-acetoxy-5,6β:22,26-diepoxy-4β,24β,25α,26(α,β)-tetrahydroxyergost-3β-methoxy-1-one (3a/3b), 15α-acetoxy-5,6β:22,26-diepoxy-4β,24β,25α,26(α,β)-tetrahydroxyergost-2-en-1-one (4a/4b), and physapubescin D (5), by spectroscopic data analysis. Among them, metabolites2 and3 are new. All of these fungal strains showed the ability to be highly stereo- and region-specific for the bioconversion of substrate (1). Our research provides a reference for the structural derivatization of withanolides or possibly even other natural products.
Source: Journal of Natural Medicines - Category: Drugs & Pharmacology Source Type: research

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Source: Journal of Asian Natural Products Research - Category: Complementary Medicine Authors: Source Type: research
In this study, we show that CRISPR/Cas9 can also be used to efficiently change functional properties of the proteins encoded by the target gene by on-site genomic mutations in A. niger. The obtained strains with constitutively active XlnR and GaaR versions resulted in increased production of plant biomass degrading enzymes and improved release of D-xylose and L-arabinose from wheat bran, and D-galacturonic acid from sugar beet pulp.
Source: Enzyme and Microbial Technology - Category: Biotechnology Source Type: research
Publication date: Available online 25 January 2020Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Filipe Soares Bertges, Maria da Penha Henriques do Amaral, Mirian Pereira Rodarte, Maria José Vieira Fonseca, Orlando Vieira Sousa, Fernanda Maria Pinto Vilela, Maria Silvana AlvesAbstractGreen coffee beans (GCB) (Coffea arabica L.) contain phenolic compounds with proven antioxidant capacity. GCB fermentation by Aspergillus oryzae was focused on the biotransformation of phenolic compounds into smaller molecules targeting a greater skin penetration. GCB was fermented under solid state for 24, 36, and 48 h...
Source: Biocatalysis and Agricultural Biotechnology - Category: Biotechnology Source Type: research
Source: Journal of Asian Natural Products Research - Category: Complementary Medicine Authors: Source Type: research
Rezafungin (RZF) is a novel echinocandin in development for prevention of invasive fungal disease caused by Candida, Aspergillus, and Pneumocystis species in patients undergoing blood and marrow transplantation. RZF has a favorable safety and tolerability profile, a low risk of drug-drug interactions and with its stability, pharmacokinetics that allow for once-weekly dosing with broad distribution to the lung and other fungal target organs.We previously demonstrated RZF efficacy in preventing Pneumocystis infection in an immunosuppressed mouse model.
Source: Biology of Blood and Marrow Transplantation - Category: Hematology Authors: Tags: 502 Source Type: research
AbstractIn a search for novel therapeutic agents against pathogenic fungal species,Candida in addition to bacterial species, novel spinel nanoferrites were assayed against four pathogenic fungi isolated from different clinical samples of ear and skin infections:Aspergillus flavus, A. niger, A. terrus and A. fumigatus, fourCandia species:Candida albicans, C. parapsilosis, C. krusei andC. tropicales, and four bacterial species: two Gram +ve:Bacillus subtilis andStreptococcus pyogenes, and two Gram −ve:Pseudomonas vulgaris andEscherichia coli. It was found that the assayed compounds displayed different levels of antifun...
Source: World Journal of Microbiology and Biotechnology - Category: Microbiology Source Type: research
AbstractOchratoxin A is one of the most diffused mycotoxin present in a large spectrum of food commodities, mainly produced byAspergillus ochraceus,Aspergillus carbonarius,Aspergillus niger andPenicillium verrucosum. EU has set maximum limits for a number of matrices such as cereals, wine, spices and liquorice, whilst other commodities such as beer and meat products that are susceptible of OTA contamination and are largely consumed are not included. In 2013, within the framework of the Regulation (EC) 882/2004 on official controls, the European Commission issued the mandate M/520 regarding the standardisation for methods o...
Source: Mycotoxin Research - Category: Toxicology Source Type: research
Publication date: Available online 22 January 2020Source: Journal de Mycologie MédicaleAuthor(s): Arghadip Samaddar, Anuradha Sharma, Twishi ShrimaliAbstractAcrophialophora fusispora is a soil-borne fungus rarely implicated in human infections. Here, we report a case of pulmonary infection due to A. fusispora in a 59-year-old male who presented with productive cough and gradually progressive dyspnoea for 20 days. He had a past history of pulmonary tuberculosis and was a known case of chronic obstructive pulmonary disease for past five years. He was diagnosed with mixed connective tissue disease and had been receivin...
Source: Journal of Medical Mycology - Category: Biology Source Type: research
"Clinical Aspects of Chronic Granulomatous Disease in Upper Egypt". Immunol Invest. 2020 Jan 22;:1-13 Authors: El-Mokhtar MA, Salama EH, Fahmy EM, Mohamed ME Abstract Chronic granulomatous disease (CGD) is a rare inherited primary immunodeficiency disorder that affects phagocytes and is characterized by a marked increased susceptibility to severe bacterial and fungal infections. We aimed to describe the clinical presentations of pediatric patients with CGD in Upper Egypt and to identify the defective component of NADPH oxidase. Pediatric patients diagnosed with CGD within one year from Januar...
Source: Immunological Investigations - Category: Allergy & Immunology Tags: Immunol Invest Source Type: research
Simultaneous detection technology has become a hot topic in analytical chemistry; however, very few reports on how to simultaneously detect small molecular contaminants and microorganisms have been in place. Aflatoxins are a group of highly toxic and carcinogenic compounds, which are produced mainly by Aspergillus flavus and Aspergillus parasiticus from section Flavi responsible for aflatoxin accumulation in stored cereals. Both aflatoxins and Aspergillus section Flavi were used to demonstrate the duplex real-time RCR method of simultaneously detecting small molecular contaminants and microorganisms. The detection of aflat...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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