Evaluation of Immunochromatography Tests and New Enzyme Immunoassay for Detection of Novel GII.17 Norovirus in Stool Samples.

In this study, the sensitivity of seven brands of immunochromatography (IC) test kits, 6 are on the market in Japan and 1 in Europe, were evaluated by testing with confirmed GII.17 NoV positive stool samples. The stool samples were also tested by bioluminescent enzyme immunoassay (BLEIA). Real-time RT-PCR was used as a gold standard method. Among seven brands IC kits, RIDA QUICK, was the most sensitive kit with the detection rate of 10(7) copies/ml while those of other IC kits ranged from 10(8)-10(9) copies/ml. Nevertheless, it should be pointed out that the detection rate of BLEIA was about 100-1,000 times (10(4)-10(5) copies/ml) higher than those of RIDA QUICK. However, the procedure for BLEIA took a longer time and required sophisticated equipment. PMID: 28003602 [PubMed - as supplied by publisher]
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research