Determination of phenolic compounds and diterpenes in roots of Salvia miltiorrhiza and Salvia przewalskii by two LC –MS tools: Multi-stage and high resolution tandem mass spectrometry with assessment of antioxidant capacity

Publication date: Available online 6 December 2016 Source:Phytochemistry Letters Author(s): Marcin Ożarowski, Anna Piasecka, Agnieszka Gryszczyńska, Aneta Sawikowska, Aurelia Pietrowiak, Bogna Opala, Przemysław Ł. Mikołajczak, Radosław Kujawski, Piotr Kachlicki, Waldemar Buchwald, Agnieszka Seremak-Mrozikiewicz Comparative phytochemical analyses of hydroalcoholic (50% EtOH) extracts from roots of S. miltiorrhiza (SM) and S. przewalskii (SP) were performed using two complementary LC–MS systems: the first system HPLC-DAD-MSn an ion trap mass spectrometer and the second system consisted high resolution MS/MS Orbitrap mass spectrometer. The individual compounds were identified using a previously published approach via comparison of the exact molecular masses, mass spectra and retention times to those of standard compounds, online available databases and literature data. Moreover, the determination of antioxidative activities of extracts by DPPH and FRAP methods was carried out. Analysis allowed to identify 39 chemical compounds in extracts from both species. Extract from root of SP differs from SM in the presence of several metabolites such as: przewalskinic acid and their derivatives, przewaquinone C, przewaquinonate A, glycosides of rosmarinic acid, methyltanshinonate, whereas tanshinones, salvianolic acids and lithospermic acids occurred in both species. Moreover, it was shown that hydroalcoholic extract from roots of SM exerted stronger antioxidant proper...
Source: Phytochemistry Letters - Category: Chemistry Source Type: research