Processes for Producing and Purifying Nucleic Acid-Containing Compositions

This technology consists of improved processes for producing and purifying nucleic acid-containing compositions, such as non-naturally occurring viruses, for example, recombinant polioviruses that can be used as oncolytic agents. Some of the improved processes relate to producing viral DNA templates and for chromatographic purification of nucleic acid-containing compositions, in which the nucleic acid is quantified in chromatography fractions with the rapid detection of one or more nucleic acid sequences (e.g., using real time RT-qPCR detection). In addition, the invention includes improved processes for production and purification of oncolytic poliovirus, such as PVSRIPO. Compositions generated using these methods are also described.IC: NCINIH Ref. No.: E-267-2014/0Advantages: Cost- and time-effective means of producing highly purified virus-based GMP products, such as oncolytic viruses, for regulatory approval.Applications: Large-scale manufacturing for producing highly purified, live virus.Improved viral purification process that increases the yield and/or purity of the resulting product, while decreasing the purification time; is generally applicable to purification of any nucleic acid molecule-containing composition, such as virus-based composition, and can be used for the purification of live native or recombinant viruses necessary for clinical applications.Improved process for generating viral template plasmid (such as one that includes a DNA templa...

Source: NIH OTT Licensing Opportunities - August 25, 2016 Category: Research Authors: ajoyprabhu3 Source Type: research