Development of a dose-limiting data collection strategy for serial synchrotron rotation crystallography

In this study, mercury single-wavelength anomalous diffraction (Hg-SAD) phasing of the luciferin regenerating enzyme (LRE) was performed using serial synchrotron rotation crystallography. The advantages of rotation and influence of dose on the data collected were evaluated. The results showed that sample rotation was effective for accurate data collection, and the optimum helical rotation step depended on multiple factors such as multiplicity and partiality of reflections, exposure time per rotation angle and the contribution from background scattering. For the LRE microcrystals, 0.25 ° was the best rotation step for the achievable resolution limit, whereas a rotation step larger than or equal to 1 ° was favorable for Hg-SAD phasing. Although an accumulated dose beyond 1.1   MGy caused specific damage at the Hg site, increases in resolution and anomalous signal were observed up to 3.4   MGy because of a higher signal-to-noise ratio.

Source: Journal of Synchrotron Radiation - November 28, 2016 Category: Physics Authors: Hasegawa, K. Yamashita, K. Murai, T. Nuemket, N. Hirata, K. Ueno, G. Ago, H. Nakatsu, T. Kumasaka, T. Yamamoto, M. Tags: serial synchrotron crystallography mercury single-wavelength anomalous diffraction radiation damage research papers Source Type: research