Affinity Purification Combined with Mass Spectrometry to Identify Herpes Simplex Virus Protein–Protein Interactions

The identification and characterization of herpes simplex virus protein interaction complexes are fundamental to understanding the molecular mechanisms governing the replication and pathogenesis of the virus. Recent advances in affinity-based methods, mass spectrometry configurations, and bioinformatics tools have greatly increased the quantity and quality of protein–protein interaction datasets. In this chapter, detailed and reliable methods that can easily be implemented are presented for the identification of protein–protein interactions using cryogenic cell lysis, affinity purification, trypsin digestion, and mass spectrometry.
Source: Springer protocols feed by Infectious Diseases - Category: Infectious Diseases Source Type: news