Constituents from Vitex negundo var. heterophylla and their inhibition of nitric oxide production

AbstractAn iridoid glucoside, 10-p-hydroxybenzoyl-6β-hydroxyiridoid 1-O-β-d-(6 ′-O-p-hydroxybenzoyl)-glucopyranoside (1), and a phenol glucoside, 4-hydroxyphenethanol 3-O-β-d-(6 ′-O-p-hydroxybenzoyl)-glucopyranoside (2), along with nine known compounds (3–11) were isolated from the dried leaves ofVitex negundo var.heterophylla. Their structures were elucidated by extensive analysis of NMR spectra. All of the isolated compounds were evaluated for their inhibitory effects on nitric oxide production in RAW 264.7 macrophages. Compounds2 and3 exhibited obvious inhibitory effects on NO production with IC50 values of 30.76 and 49.89  μM, respectively. Molecular docking studies of compounds2 and3 with nitric oxide synthase (NOS) further confirmed the above results.
Source: Journal of Natural Medicines - Category: Drugs & Pharmacology Source Type: research