Accurate Sample Assignment in a Multiplexed, Ultrasensitive, High-Throughput Sequencing Assay for Minimal Residual Disease
High-throughput sequencing (HTS) (next-generation sequencing) of the rearranged Ig and T-cell receptor genes promises to be less expensive and more sensitive than current methods of monitoring minimal residual disease (MRD) in patients with acute lymphoblastic leukemia. However, the adoption of new approaches by clinical laboratories requires careful evaluation of all potential sources of error and the development of strategies to ensure the highest accuracy. Timely and efficient clinical use of HTS platforms will depend on combining multiple samples (multiplexing) in each sequencing run.
Source: Journal of Molecular Diagnostics - Category: Pathology Authors: Jack Bartram, Edward Mountjoy, Tony Brooks, Jeremy Hancock, Helen Williamson, Gary Wright, John Moppett, Nick Goulden, Mike Hubank Tags: Regular article Source Type: research