SREBP Inhibition Ameliorates Renal Injury after Unilateral Ureteral Obstruction.

SREBP Inhibition Ameliorates Renal Injury after Unilateral Ureteral Obstruction. Am J Physiol Renal Physiol. 2016 Jul 6;:ajprenal.00140.2016 Authors: Mustafa M, Wang TN, Chen X, Gao B, Krepinsky JC Abstract Tubulointerstitial fibrosis is a major feature associated with declining kidney function in chronic kidney disease of diverse etiology. No effective means as yet exists to prevent the progression of fibrosis. We have shown that the transcription factor SREBP-1 is an important mediator of the profibrotic response to TGFβ and Angiotensin II, both key cytokines in the fibrotic process. Here we examined the role of SREBP in renal interstitial fibrosis in the unilateral ureteral obstruction (UUO) model. The two isoforms of SREBP (-1 and -2) were activated by 3 days after UUO, with SREBP-1 showing a more sustained activation to 21 days. We then examined whether SREBP1/2 inhibition with the small molecule inhibitor fatostatin could attenuate fibrosis after 14 days of UUO. SREBP activation was confirmed to be inhibited by fatostatin. Treatment decreased interstitial fibrosis, TGFβ signaling, and upregulation of αSMA, a marker of fibroblast activation. Fatostatin also attenuated inflammatory cell infiltrate and apoptosis. Associated with this, fatostatin preserved proximal tubular mass. The significant increase in atubular glomeruli observed after UUO, known to correlate with irreversible renal functional decline, was also decreased by treatmen...
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research

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We report outcomes of our initial experience with LP in 38 children from 2 months of age. Materials and Methods From June 2015 to December 2017 38 children aged 2-60 months (mean age 1.7 years) underwent LP for correction of PUJ obstruction. The mean pre operative anteroposterior diameter of the renal pelvis (APD) was 43,5mm and all patients had hydronephrosis (APD 21.4-76 mm) and obstructed curve on diuretic renogram. Anderson-Hynes pyeloplasty was the performed technique. Results are reported. Results Mean operative time was 107 minutes (70-180) with no conversion to open procedure. Pain control was needed mainly in the ...
Source: International Braz J Urol - Category: Urology & Nephrology Source Type: research
Prehydrating patients who have chronic kidney disease before they undergo a...Read more on AuntMinnie.comRelated Reading: Experts reaffirm safety of contrast use for kidney CT Could patient history help predict CT contrast reactions? Oral contrast not needed to spot kids' appendicitis on CT Contrast use doesn't change acute kidney injury rate CT contrast not tied to poor outcomes, even in at-risk patientsComments: 2/18/2020 6:19:39 PMChris Woodhouse You guys are driving me crazy!  Just when I get the clinic settled into the practice of pushing clear fluids before any contrast study, you tell me it doesn't ...
Source: Headlines - Category: Radiology Source Type: news
Maternal endotoxemia induces renal collagen deposition in adult offspring: Role of NADPH oxidase/TGF-β1/MMP-2 signaling pathway. Arch Biochem Biophys. 2020 Feb 17;:108306 Authors: Farias JS, Santos KM, Lima NKS, Cabral EV, Aires RS, Veras AC, Paixão AD, Vieira LD Abstract Maternal endotoxemia has been shown to increase renal collagen deposition in the offspring. Renal fibrosis is a hallmark of progressive chronic kidney disease. It was investigated whether maternal reactive oxygen species (ROS) leads to renal fibrosis or exacerbates unilateral ureteral obstruction (UUO)-induced renal fibro...
Source: Archives of Biochemistry and Biophysics - Category: Biochemistry Authors: Tags: Arch Biochem Biophys Source Type: research
In conclusion, hirudin depressed the fibrosis in renal tissues and renal tubular epithelial cells by inhibiting the inflammation, regulating the related proteins of fibrosis and ETM and decreasing the apoptosis of renal tubular epithelial cells. These findings may offer an effective treatment method for RIF. PMID: 32066115 [PubMed - as supplied by publisher]
Source: International Immunopharmacology - Category: Allergy & Immunology Authors: Tags: Int Immunopharmacol Source Type: research
AbstractProgressive tubulointerstitial fibrosis is the common final outcome for all kidney diseases evolving into chronic kidney disease (CKD), whereas molecular mechanisms driving fibrogenesis remain elusive. Retinoic acid-inducible gene-I (RIG-I), an intracellular pattern recognition receptor, is originally identified participating in immune response by recognizing virus RNA. Here, we revealed for the first time that RIG-I was induced in unilateral ureteral obstruction (UUO) and folic acid (FA) renal fibrosis models and moderate-degree renal fibrosis patients. Besides, we found RIG-I was mainly located in renal tubular e...
Source: Journal of Molecular Medicine - Category: Molecular Biology Source Type: research
This study reports for the first time that OI ameliorated renal fibrosis by suppressing the activation of TGF-β/Smad and nuclear factor kappa B (NF-κB) pathways, reducing generation of reactive oxygen species and inhibiting autophagy. These results clearly suggest that OI has great clinical potential for managing renal fibrosis. PMID: 32032597 [PubMed - as supplied by publisher]
Source: European Journal of Pharmacology - Category: Drugs & Pharmacology Authors: Tags: Eur J Pharmacol Source Type: research
Conclusion: SSB2 could attenuate renal fibrosis and decrease fibroblast activation by inhibiting the Hh signaling pathway.Graphical Abstract
Source: Phytomedicine - Category: Drugs & Pharmacology Source Type: research
In conclusion, the therapeutic effects of H2S on UUO-induced renal injury and fibrosis are at least in part by inhibition of M1 and M2 macrophage infiltration. H2S suppresses NLRP3 activation and subsequently inactivates NF-κB and IL4/STAT6 signaling, which may contribute to the anti-inflammatory and anti-fibrotic effects of H2S. PMID: 31846625 [PubMed - as supplied by publisher]
Source: Experimental Cell Research - Category: Cytology Authors: Tags: Exp Cell Res Source Type: research
In this study we investigated whether GA exerted antirenal fibrosis effect in a unilateral ureteral obstruction (UUO) mouse model. After UUO surgery, the mice were treated with GA (3.125, 12.5, and 50 mg· kg-1 ·d-1, ip) for 7 or 14 days. Then the mice were sacrificed for collecting blood and kidneys. We showed that GA treatment dose-dependently attenuated UUO-induced tubular injury and renal fibrosis; GA (50 mg· kg-1 ·d-1) significantly ameliorated renal disfunction during fibrosis progression. We further revealed that GA treatment inhibited the extracel...
Source: Acta Pharmacologica Sinica - Category: Drugs & Pharmacology Authors: Tags: Acta Pharmacol Sin Source Type: research
A 34 year-old female with Stage V chronic kidney disease secondary to a history of sickle cell anemia and focal segmental glomerulosclerosis and on peritoneal dialysis presented to our hospital for imaging as part of routine evaluation for renal transplant. Upon receiving an MRI of the abdomen to evaluate liver architecture and kidney anatomy, a 6.6 cm left perinephric mass was incidentally found inferomedial to the left kidney and extending caudally (Figure 1). The renal parenchyma and ureter were uninvolved.
Source: Urology - Category: Urology & Nephrology Authors: Tags: Grand Rounds Source Type: research
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