Development and characterization of 26 polymorphic microsatellite markers in Lateolabrax maculatus and cross-species amplification for the phylogenetically related taxa

In this study, we developed 26 polynucleotide-repeat microsatellite loci for L. maculatus through high-throughput DNA sequencing technology. The number of alleles per locus ranged from 3 to 19. Observed and expected heterozygosities varied from 0.188 to 0.906 and 0.175 to 0.938, respectively. One locus showed significant deviation from Hardy-Weinberg equilibrium after Bonferroni correction. These 26 novel microsatellite markers will serve as useful tools which benefit population genetics studies and conservation management of L. maculatus. In addition, cross-species amplification tests showed that the transferability of 6 loci was positive across 10 species of two phylogenetically related families Latidae and Serranidae, and 3 loci were successful amplified in at least 5 species. The transferable loci would be helpful for the detection of genetic relationship and divergence between the tested taxa.

Source: Biochemical Systematics and Ecology - May 25, 2016 Category: Biochemistry Source Type: research