Epithelial p85 and p53 Regulate Survivin Expression during Adaptation to Ileocecal Resection.

Epithelial p85 and p53 Regulate Survivin Expression during Adaptation to Ileocecal Resection.
Am J Pathol. 2016 May 5;
Authors: Cohran V, Managlia E, Bradford EM, Goretsky T, Li T, Katzman RB, Cheresh P, Brown JB, Hawkins J, Liu SX, De Plaen IG, Weitkamp JH, Helmrath M, Zhang Z, Barrett TA
Abstract
Intestinal adaptation to small-bowel resection (SBR) after necrotizing enterocolitis expands absorptive surface areas and promotes enteral autonomy. Survivin increases proliferation and blunts apoptosis. The current study examines survivin in intestinal epithelial cells after ileocecal resection. Wild-type and epithelial p85α-deficient mice underwent sham surgery or 30% resection. RNA and protein were isolated from small bowel to determine levels of β-catenin target gene expression, activated caspase-3, survivin, p85α, and p53. Healthy and post-resection human infant small-bowel sections were analyzed for survivin, Ki-67, and p53 by immunohistochemistry. Five days after ileocecal resection, epithelial levels of survivin increased relative to sham-operated on mice, which correlated with reduced cleaved caspase-3, p85α, and p53. At baseline, p85α-deficient intestinal epithelial cells had less p53 and more survivin, and relative responses to resection were blunted compared with wild-type. In infant small bowel, survivin in transit amplifying cells increased 71% after SBR. Resection increased proliferation and decreased numbers of p53-pos...

Source: The American Journal of Pathology - May 4, 2016 Category: Pathology Authors: Cohran V, Managlia E, Bradford EM, Goretsky T, Li T, Katzman RB, Cheresh P, Brown JB, Hawkins J, Liu SX, De Plaen IG, Weitkamp JH, Helmrath M, Zhang Z, Barrett TA Tags: Am J Pathol Source Type: research