Molecular detection and isolation of avian metapneumovirus in Mexico.

Molecular detection and isolation of avian metapneumovirus in Mexico.
Avian Pathol. 2014 Mar 12;
Authors: Rivera-Benitez JF, Martínez-Bautista R, Ríos-Cambre F, Ramírez-Mendoza H
Abstract
We conducted a longitudinal study to detect and isolate avian metapneumovirus (aMPV) in two highly productive poultry areas in Mexico. A total of 968 breeder hens and pullets from 2 to 73 weeks of age were analysed. Serology was performed to detect aMPV antibodies and 105 samples of tracheal tissue were collected, pooled by age, and used for attempted virus isolation and aMPV nested RT-PCR (nRT-PCR). The serological analysis indicated that 100% of the sampled chickens showed aMPV antibodies by 12 weeks of age. Five pools of pullet samples collected at 3 to 8 weeks of age were positive by nRT-PCR and the sequences obtained indicated 98-99% similarity to the reported sequences for aMPV subtype A. Virus isolation of nRT-PCR-positive samples was successfully attempted using chicken embryo lung and trachea mixed cultures with subsequent adaptation to Vero cells. This is the first report of detection and isolation of aMPV in Mexico.
PMID: 24617750 [PubMed - as supplied by publisher]

Source: Avian Pathology - March 12, 2014 Category: Pathology Authors: Rivera-Benitez JF, Martínez-Bautista R, Ríos-Cambre F, Ramírez-Mendoza H Tags: Avian Pathol Source Type: research