Novel Electrochemical Biosensor for Apoptosis Evaluation

Apoptosis evaluation is one of the most important tasks of toxicology. By using a peptide as the recognition element, and assembling apoptotic cells on a solid surface, we have established a novel electrochemical method for the detection of apoptosis levels. Such a peptide-based electrochemical biosensor is simple, cost-effective, convenient, and sensitive. Since the results obtained are well in line with other standard methods, this method holds a great potential towards the analysis of apoptosis and its applications. In this chapter, we introduce a general overview of this technical approach for detecting apoptotic cells. We discuss its advantages over the ordinary methods. We also provide practical guidelines for designing studies, and summarize the step-by-step protocols used in our lab for sample preparation, electrode modification, and accurate electrochemical quantification of apoptotic cells.
Source: Springer protocols feed by Pharmacology/Toxicology - Category: Drugs & Pharmacology Source Type: news