Southern Blotting Confusion

Researchers conducted an experiment by comparing DNA samples from 3 individuals: homozygote for the normal ß globin gene, homozygote for mutant sickle cell allele and heterozygote for a carrier. Each DNA sample is mixed with the same restriction enzyme, DdeI. Digestion leads to thousands of fragments. Gel electrophoresis is conducted forming a characteristic of bands and treated later with a stain. The gel is transferred and via capillary action the alkaline solution is pulled through the... Southern Blotting Confusion
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