Southern Blotting Confusion
Researchers conducted an experiment by comparing DNA samples from 3 individuals: homozygote for the normal ß globin gene, homozygote for mutant sickle cell allele and heterozygote for a carrier.
Each DNA sample is mixed with the same restriction enzyme, DdeI. Digestion leads to thousands of fragments. Gel electrophoresis is conducted forming a characteristic of bands and treated later with a stain. The gel is transferred and via capillary action the alkaline solution is pulled through the...
Southern Blotting Confusion
Source: Student Doctor Network - Category: Universities & Medical Training Authors: betterfuture Source Type: forums