A novel approach for next‐generation sequencing of circulating tumor cells

ConclusionThese spiking experiments provide proof of concept of a clinically applicable workflow for real‐time monitoring of patient tumor using noninvasive liquid biopsies. The application of solid tumor next‐generation sequencing (NGS) approaches to circulating tumor samples has been hampered by the low‐input DNA available from rare circulating tumor cells (CTCs), and whole genome amplification (WGA) approaches used to generate sufficient input DNA are often incompatible with blood collection tube preservatives used to facilitate clinical sample batching. We developed a novel approach combining tumor cell isolation from preserved blood with Repli‐G WGA and Illumina TruSeq Amplicon Cancer Panel‐based NGS. We applied this approach to purified cell pools ranging from 10 to 1000 cells from three different cell lines to provide proof of concept of a clinically applicable workflow for real‐time monitoring of patient tumor using noninvasive liquid biopsies.
Source: Molecular Genetics & Genomic Medicine - Category: Genetics & Stem Cells Authors: Tags: Method Source Type: research