Characterization, prediction and evolution of plant peroxisomal targeting signals type 1 (PTS1s)

Publication date: Available online 6 January 2016 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): S. Reumann, G. Chowdhary, T. Lingner Our knowledge of the proteome of plant peroxisomes and their functional plasticity is far from being complete, primarily due to major technical challenges in experimental proteome research of the fragile cell organelle. Several unexpected novel plant peroxisome functions, for instance in biotin and phylloquinone biosynthesis, have been uncovered recently. Nevertheless, very few regulatory and membrane proteins of plant peroxisomes have been identified and functionally described up to now. To define the matrix proteome of plant peroxisomes, computational methods have emerged as important powerful tools. Novel prediction approaches of high sensitivity and specificity have been developed for peroxisome targeting signals type 1 (PTS1) and have been validated by in vivo subcellular targeting analyses and thermodynamic binding studies with the cytosolic receptor, PEX5. Accordingly, the algorithms allow the correct prediction of many novel peroxisome-targeted proteins from plant genome sequences and the discovery of additional organelle functions. In this review, we provide an overview of methodologies, capabilities and accuracies of available prediction algorithms for PTS1 carrying proteins. We also summarize and discuss recent quantitative, structural, and mechanistic information of the interaction of PEX5 ...

Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 11, 2016 Category: Molecular Biology Source Type: research