Pseudomonas aeruginosa flagellin as an adjuvant: The superiority of the conjugated form of flagellin versus the mixed one to an HIV-1 vaccine candidate in the induction of immune responses.

Pseudomonas aeruginosa flagellin as an adjuvant: The superiority of the conjugated form of flagellin versus the mixed one to an HIV-1 vaccine candidate in the induction of immune responses.
J Med Microbiol. 2015 Sep 23;
Authors: Delavari S, Sohrabi M, Shafiee Ardestani M, Faezi S, Tebianian M, Taghizadeh M, Shajiei A, Hosseini SY, Moghaddampour M, Mahdavi M
Abstract
In the present study, the adjuvant activity of flagellin was compared in the conjugated and mixed forms, against a peptide vaccine from HIV-1 p24-Nef vaccine candidate. The mice were immunized with the HIV-1 p24-Nef peptide with flagellin in both conjugated and mixed forms. Lymphocyte proliferation, CTL activity, and IL-4 and IFN- cytokines was evaluated by Brdu, CFSE, and ELISA methods, respectively. At the same time, the frequency of IFN- producing T lymphocytes as well as total and isotype-specific antibodies were assessed by ELISPOT and indirect ELISA, respectively. Our experimental results showed that the immunized mice with the HIV-1 p24-Nef conjugated or mixed forms of flagellin lead to increases in the proliferative responses and Th1 cytokine pattern. The conjugated form of vaccine led to increased CTL activity, Th1 cytokine pattern of immune responses as well as IgM isotype of humoral responses in comparison with mixed form. The flagellin-conjugated vaccine seems to be more potent in increasing vaccine immunogenicity.
PMID: 26404477 [PubMed - as sup...

Source: Journal of Medical Microbiology - September 23, 2015 Category: Microbiology Authors: Delavari S, Sohrabi M, Shafiee Ardestani M, Faezi S, Tebianian M, Taghizadeh M, Shajiei A, Hosseini SY, Moghaddampour M, Mahdavi M Tags: J Med Microbiol Source Type: research