GSE265958 Deletion of an sRNA primes development in a multicellular bacterium

Contributors : Marco La Fortezza ; Jasper Verwilt ; Sarah Cossey ; Sabrina Eisner ; Gregory J Velicer ; Yuen-Tsu Nicco YuSeries Type : Expression profiling by high throughput sequencingOrganism : Myxococcus xanthusSmall non-coding RNAs (sRNAs) are essential in regulating gene expression during many biological processes. In myxobacteria, the gene pxr encodes for an sRNA known to block the activation of fruiting body development, an aggregative multicellular process triggered by starvation. However, a comprehensive list of potential targets of Pxr and whether these are indeed associated with development still needs to be determined. Here, we profiled the transcriptome of vegetative cells lacking pxr and obtained a list of genes affected by the absence of this sRNA. Interestingly, we found that the absence of pxr influences the expression of genes associated with metabolic processes in general. However, our experiment strengthened the association between pxr and aggregative development with more than half of the differentially expressed genes linked to fruiting body formation. By ours with previously published data, we identified critical factors of myxobacteria aggregative multicellularity as potential pxr targets combining. Moreover, our study supports the existence of additional not yet described genes relevant to fruiting body development that Pxr possibly controls. Among them, we identify pxrA, a novel gene encoding for a GNAT-acetyltransferase that we showed to be imp...

Source: GEO: Gene Expression Omnibus - May 6, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Myxococcus xanthus Source Type: research