A Naphthalimide based "Turn-ON" probe for wash-free imaging of lipid-droplet in living cells with an excellent selectivity

Chembiochem. 2024 Apr 29:e202400270. doi: 10.1002/cbic.202400270. Online ahead of print.ABSTRACTThe impacts of dimethylation of 4-Amino-1,8- Naphthalimide (ANI) on its photophysical properties are reported. The resulting 4-DiMe-ANI displays completely different fluorescence properties, conferring it ability to selectively label lipid droplets in living cells. A comprehensive photophysical study revealed that this selectivity arises from an Internal Charge Transfer favored in lipophilic media to the detriment of a non-emissive TICT in more polar media. This results in a very high "LDs/Cytosol" signal ratio, enabling LDs to be imaged with an excellent signal-to-noise ratio, and positioning its performance above that of the BODIPY 493/503 commonly used to image LDs.PMID:38683177 | DOI:10.1002/cbic.202400270
Source: Chembiochem - Category: Biochemistry Authors: Source Type: research
More News: Biochemistry | Study